酵母中coiled-coil domains的系統(tǒng)研究.pdf

1、礦108’9811分類號(hào)掬咐幺害密繳研究生學(xué)位論文論文題目(中文)醛豎生￡Qi!翊：￡Qil血坐壘i墅鮑丕統(tǒng)班窒論文題目(步h文)曼Y鯉望!壘!i￡壘壁望!YiQ￡￡Qi!!墮：￡Qi!墮Q堡壘i娶i塾墜堅(jiān)魚亟i墜2Y壁壘墨!研究生姓名王勇強(qiáng)學(xué)科、專業(yè)生物堂動(dòng)物堂研究方向筮直生物堂學(xué)位級別亟導(dǎo)師姓名、職稱王王仨教攫夔態(tài)鑾硒究雖論文工作起止年月星QQ壘生旦月至星Q魚!生墨旦論文提交日期2QQZ生壘且一論文答辯日期圣QQ!生晝旦學(xué)位授予日期

2、校址：甘肅省蘭州市蘭州大學(xué)碩士學(xué)位論文Systematicanalysisofcoiled—coildomainsinbuddingyeastAbstractEukaryoficproteinsarensuallyconstructedinacaste—likefashionusingdomainsandmotifsasbuildingblocksthatmediatespecificmolecularinteractionsAmong

3、suchdomains，thecoiledcoilhasbeenfrequentlyfoundinmediatingproteinproteininteractionsToanalyzetheinteractionspecificitybctxvecncoiledcoils，wedevelopedamulti—stepstrategythatcombinescomputationandexperimenttoidentifyspecif

4、iccoiledcoilinteractionsInstep1ailcoiledcoilswerepredictedfortheproteomeofsacchromysescerevisiaenlcproteinsthatcontaincoiledcoils戤denotedaseoiledeoilproteinsInstep2acoiled—coilproteininteractionnetworkWaSobtainedbyonlyco

5、nsideringinteractionsbctweencoiled七oilproteinsintheknownproteinproteininteractionsinyeastInstep3，acomputationalprogrambasedonSVMwasusedtopredictthepotentialcoiledcoilInteractionsInstep4。thepote】atialcoiledcoilpairsweresu

6、bjecttoayeasttwohybridassayIntotal，weanalyzedtheinteractionsfor4149coiledcoilpairs478ofthemarepositivesinvoIving202coiledcoildomainsFurthermore，weattemptedtoexplorethefunctionalroleofcoiledcoilsWhenacoilcdcoilthatiSinvol

7、vedinmethionemetabolicpathwaysWasknockedout，yeastexhibitedmethionineauxotrophindicatingtheessentialroleofcoiledcoilinsuchbiologicalpathwayMeanwhile，wedevelopnewtechniquestosystematicallydetectcoiledeoilinteractionsinvivo