根際細菌鐵載體化感作用研究.pdf

1、中國農業(yè)大學碩士學位論文根際細菌鐵載體化感作用研究姓名：謝小軍申請學位級別：碩士專業(yè)：植物營養(yǎng)指導教師：王敬國2002.5.1中國農業(yè)大學碩士學位論文2002AbstractInthisstudywestartedwithscreeningofsoilbacterialisolatesgrowniniron—limitedmediafortheproductionofsiderephoresinhibitorytoplants，andt

2、heresultshowedfourstrainsofbacteriahadinhibitoryeffectonwheatwhichmightrelatetotheirsiderophore：productionQuantitativedeterminationshowedtheirgreatcapabilitytoexcretesiderophoreThesefourstrainswereidentifiedasErw加婦sp二Bya

3、seriesofchemicalandphysicalassaysuchasAmowassayCsakyassayandUVSpectrophotometricscan，eatecholor23dihydroxyhenzoicacid(2，3DHBA)wasidentified85theconstituentofsiderophoreOnthisbasis5catecholatesiniron—limitedculturesupemat

4、ant，including23一DHBAanditsderivativesfunctioningassiderophore，wereconfirmedwiththinlayerchromatography(TLC)onsilicaGandpolyamideAcatecholtypesiderophorewasp塒fiedbyethylacetateextraction，XAD4resinandpolyamidecolumnchromat

5、ographyAnalysisbyTLC，paperchromatographyandUVSpectrephotometricstallconfirmedthestrictureofpurifiedsiderophore，whichmightbeaLeuandValsubstituted23一dihydroxybenzoicacidsiderophoreThebiotictestof23DHBAandpurifiedsiderophor

6、eshowedtheyhadthesinificantlyinhibitoryeffectontheradiclegrowthofwheatandrice，andwhentheircoucentrationsreached500“gml。and200laMrespectivelytheroottipsoftheplantsappearedblackandwiltedKeywords：Allelopathy，Siderophore，23D