杜氏鹽藻在脅迫條件下的基因表達系列分析（SAGE）.pdf

1、上海大學碩士學位論文杜氏鹽藻在脅迫條件下的基因表達系列分析（SAGE）姓名：張雨申請學位級別：碩士專業(yè)：生物化工指導教師：許政暟宋任濤20060601上海大學碩士學位論文ABSTRACTDnunaliellaviridisisaunicellulargreenalgaeIthasallexcellentadaptationcapabilitytoenvironmentsInadditiontotoleratingdramaticchan

2、geoftemperature，itCallliveinthewiderangevaryingbetween50mMand5MNacl(saturated1ThesecharacterssuggestthattherearegenesandregulationmechanisminresponsetotoleratingstressinDunaliellaSo，tostudyDnaliellaandrevealitsmechanismo

3、ftoleratingstressprovidesfoundationforimprovingcrops’propertiesThesalttolerancemechanismiscontrolledbyseveralgenesandisquitecomplexThustostudysinglegeneisnotenoughandt0averttothewholegenomictransoriptomreseachisdipensabl

4、eSerialAnalysisofGeneExpression(SAGE)isasortofmolecularbioteehnologyfortranscriptome，withhi曲effeciencyIntheexperimentsevenSAGElibrariesareconstructedTheselibrariesrepresentthegeneexpressionprofilingofDunaliellaviridisins

5、evenconditions，Includingoptimumhyposmosis，hyperosmosis，hyposmoticshockhyperosmoticshockcoldshockandheatshock。ByPC＆theaverageinsertionoftheselibrariesis400bp，and90％clonesofthelibrarieshaveinsertionFiveSAGElibraries：hyposm

6、osis，hyperosmosis，hyposmoticshockhyperosmoticshockandheatshockhavebeensequencedandanalyzedbytheSAGEsoftwareTotallythereare76，558，90，206，76，204，99，010and63，930tags，Drawingacomparisonbetweenwithhyposmosisandhyperosmosis，3，

7、581genesarehi曲saltinducedand2，646genesarelowsaltinduced348tagshaveextraordinarydifferentoccurringfrequency，amountingtD5timesInthesetags175tagsaleobviouslyhi曲saltinducedwhile173tagsalelowsalt—inducedComparedwith0111“datab

8、aseofDun／／e／／aESTthetagswithhi醢expressionlevelsrepresentgenesencodingribosomalprotein；thioredoxin；ferredoxin；NADHUQreductaseandsoonWhilethetagswithobviouslydifferentoccurringfrequencyindicategenesencodingDNAbInaingprotei