霍山石斛人工種子包埋配方的優(yōu)化和萌發(fā)的研究.pdf

1、安徽農(nóng)業(yè)大學(xué)碩士學(xué)位論文霍山石斛人工種子包埋配方的優(yōu)化和萌發(fā)的研究姓名：秦自清申請學(xué)位級別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：林毅20080617TheresearchongerminationandoptimizationofembedmentofartificialseedsofDendrobiumhuoshanenseAbstractDendrobiumhuoshaneseCZTangetSjchengistreasureinmedici

2、nalDendrobium，producedinHuoshanCounty，AnhuiProvincemgrowthofitdemandsextremelyharshconditions，andseedmultiplicationcapacityisverylowduetoexcessiveexploitation，naturalresourceshavebeentialedoffitisdifficulttocarryoutlarge

3、scaleartificialcultivationforthetransplantoftesttubeplantletsofDhuoshanesehavealowersurvivalrateAtpresent，therearehavemoreofreportinartificialseeds，butartificialseedsofDhuoshanesehavenotbeenreported，therefore，wecanconsid

4、ertheadoptionofrapidpropagationofartificialseedsasaeffectivewaytosolvinglackofresourcesInthispaper，theartificialseedsofDhuoshanesewasproducedwithAuxiliarybuds，Protocorm—likebodies，andadventitiousshootsByusingofsinglefact

5、orandorthogonalexperiment，studiedontheeffectof5factors(Maltose(％)，hormoneratebetween6BA(mgL1)andNAA(mg’L。1)，activecarbon(％)，sodiumalginate(％)，timeofionexchange(rain))ongerminationrateofartificialseedsofDhuoshanese，theeff

6、ectofthetypeandsizeofpropagatorsongerminationrateandrateofseedlingsThentheleakingrateofmaltoseandvariationofpHvalueofartificialseedcapsuleduringvegetatingofartificialseedsofDhuoshanesewasmeasuredalsowestudiedontheseedlin

7、gplantsgrowthcharacteristics，artificialseedstorage，preservation，andotheraspectsinordertoprovidetechnicalmethodsfordepthstudytoartificialseedsDhuoshanese111emainresultsofstudywerefollowed：1Fromsingle—factortestthat，themal

8、toseissuitablecarbonappropriateconcentrationis4O％，byorthogonaldesigntesting，MaltoseplaysthemostimportantroleininducingtheDhuoshaneseartificialseedstogerminate，themostappropriatecombinationis：maltose40％，hormoneratebetween

9、6BA(mg。L1)andNAA(nag‘L1)10：1，activecarbon01％，sodiumAlginate4％，timeofionexchangeis10rainEmbeddedinthisformulathegerminationrateofartificialseedsofDhuoshanesetakeingauxiliarybuds，protocormlikebodies，andadventitiousshootsas