對甘藍夜蛾、粘蟲高毒力Bt菌株的篩選及其某些生物學(xué)特性的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文對甘藍夜蛾、粘蟲高毒力Bt菌株的篩選及其某些生物學(xué)特性的研究姓名：樊東申請學(xué)位級別：碩士專業(yè)：昆蟲學(xué)指導(dǎo)教師：李國勛19991101英文摘要AbstractTenBtstrainswithhig|lefficacywerescreenedfrom74Bacillusthuringiensisfro)strainswhichwereisolatedfromdeseasedlarvaeofL印idolXerousin

2、sectsandWCleusedtotesttheirtoxicityonMamestrabrassicae(L)andMythimnaseparata(W)larvebybioassay11lcresultsshowedthatthemortalityofMbrassicaetreatedwiththe5oftheStrailuatconcentrationofl6107spores／m]was80％95％n托Lc500fthe5st

3、rainsto塒：brassicaewere218X105、447X105、220X1護、20Xl礦and507X105sporesdmlseparatelyThemortalityofALseparatatreatedwith5oftllestrainsatconcentrationofl6108spores／mlwas80％95％TheLc50were549X106、243X106、447X106、460X106and356妯06s

4、eparatelyThegrowthpropertiesofthestrainswasobservedfrom12hourstO72hoursTheresultshewedthatthereⅥ㈣6differemtypesingrowthlilediumThemorpho：ogiesofparasporalbodyappeareddifferenceinshapeincludingrhomboid(71，6％Xspharialortri

5、angle(41％)，cube(27M)，irregular(81％)andmixer(135％)TenBtstraimwithhighe伍cacywereallrhomboidTheesterasepanernsfromthestrainswereanalyzedbythepolyacrylamidegelelectrophoresisFourkindefesterasepaaernsinthesestrainswereobtaine

6、dTheesterasepatternsofeightofthescreenedstrainsweresameasthatofDPand087Twostrains’esterasepatternscouldnotbeenidemifiedn∞proteinsofparasporalbodyweredeterminedbySD孓一PAGEandthemolecularweightswereealculated砒about132KDand1