飛秒激光顯微操作進行單細胞水平的蝦青素生物合成研究.pdf

1、天津大學博士學位論文飛秒激光顯微操作進行單細胞水平的蝦青素生物合成研究Studyofastaxanthinbiosynthesizeatsinglecelllevelbyfemtosecondlasermanipulation(國家自然科學基金重點項目資助NSFC一20536040)(國家自然科學基金項目資助NSFC一60578037)(國家973計劃項目資助NO2003CB716003、2007CB07802)學科專業(yè)：生物化工研究生

2、：鞏繼賢指導教師：趙學明教授天津大學化工學院二零零七年七月Abs訂actAbstractSingle—celltechniqueshavebeenusedtodescribethecompositionandactivitiesofcells，havebeenakeyelementinrevealingtherelationshipofenvironmentaldistributionandactivitiesofmicroorgani

3、sms，andhavebeenusedtodetaildynamicsofreactionsincellsSinglecellmethodshavealsobeenessentialtounderstandingproblemsinbioengineeringsuchasthescreeningofhigh—productstrainsFemtosecondlaserhasattractiveinsinglecellresearchfo

4、rhightemporalresolutionandhighspatialresolutionStudyofastaxanthinbiosynthesizeatsinglecelllevelwasperformedwithHaematococcuspluvialisandPhaffiarhodozymabyfemtosecondlasermanipulationinthisarticleThetrappingoflivingcellsb

5、yfemtosecondlasertweezerswasbeenstudiedQvalueoffemtosecondlasertweezerswasmeasuredandthetrappingcapabilityoffemtosecondlaserandcontinuouswavewascomparedTheresultprovedthatfemtosecondlasercouldbeusedasopticaltweezersaswel

6、lascontinuouslaserAdditionalaprimaryattemptwasmadeinusingopticalcrystalfiberasopticalt、ⅣeezersAsystemstudyofactivityofcellsinthetrappingoffemtosecondlasertweezerswasperformedbytheanalysisoftheeffectoflaserpowerandexposur

7、etimeoncellactivityTherangeoflaserpowerandexposuretimeforsafetrappinghasbeenprovidedThetransferofsinglecellfromthesuspensiontoplatehasbeenachievedsuccessfullyonthebaseofthetrappingbyfemtosecondlaserFemtosecondlaserpulseh

8、asbeenusedasthemethodofmutagenesisforthefnsttimeThecellsofHaematococcuspluvialisandPhaffiarhodozymahavebeentreatedwithfemtosecondlaseratsinglecelllevelTheresultsshowedthatthemutagenesisbyfemtosecondlaseratsinglecelllevel

9、succeedforHaematococcuspluvialiswhilefailedforthaffiarhodozymaAprimaryattemptwasCalTyoutindetectingofastaxanthincontentinsinglecellbymicrospectroscopicmethodThepreparationofprotoplastofHaematococcuspluvialisandPhaffiarho

10、dozymahasbeenaccomplishedthroughtheselectionofappropriateenzymeandtheanalysisofconditionsFemtosecondlaserinducedcellfusionwasachievedforthefnsttimeThefusionofPhaffiarhodozymaprotoplastwasperformedwithfemtosecondlasermani