粘細(xì)菌的分離、鑒定及活性研究.pdf

1、上海師范大學(xué)碩士學(xué)位論文粘細(xì)菌的分離、鑒定及活性研究姓名：張琦申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：袁萍20050501ABSTRACTMyxobacteriaisakindofhigherprokaryotewithsocialbehavior.Theyhavecomplicatedcooperativemotilitybehaviorandappearanceformationsotheyareimportanttotheres

2、earchofcelldiferentiationdevelopmentandevolution.Inlateyearsmyxobacteriaarevaluedfortheirlargenumberofbiologicallyactivesecondarymetabolites.Inthisresearch171myxobacteriastrainswereseparatedfrom52samplescollectedfromsoil

3、splantswaterin11areassuchasJilinShandongShanxiSichuanShanghaiZhejiangetc.and63purestainsweregot.Byobservingcoloniesmyxosporesnutrientcellsofthestrainsandabilityofdissolvingcellulosethesepurestrainsweredividedinto4types.M

4、yxobacteriausuallygrowpoorlyinliqiudmediaanddiferentstrainsneedcertainmedia.StrainP224andstrainX1wereculturedinYSEHtIRI.MEandM26liquidmedia.ItwasfoundthatthestrainP224grewbetterinMEM26andYSmediathestrainX1weresuitableinY

5、SandHtmediaThefermentationliquidsof63strainsweredetectedfortheactivitiesagainstbacteriafungiantibioticresistantbacteriaandagglutination.Theresultsshowedthat18strainsresistedStaphylococcusaureus.18strainshadactivityofanti

6、coagulation.Theyare29%ofthewholestrains.BacillussubtilisissensitivetostrainS16andS33whereasCandidaalbicansissensitivetostrainA2andA4.Thesetwokindsofbioactivestrainsare3%inthewholestrainsStrainA1withdissolvingcelluloseabi

7、lityandstrainA4whichcanresistCandidaalbicanswereclassified.AccordingtotheappearanceofnutrientcellsfruitingbodiesmyxosporesofthistwostrainsandthecoloniesinmediaST21CXWCXVY2etc.inadditiontophysiologicalcharacteristicssucha

8、scongoredreactionnitraterecoverfibrindissolvingantibioticsensitivityandGCmol%165rRNAsequencesanalysisstrainAIwasclassifiedtoPolyangiumcellulosumandstrainA4toPolyangiumfumosum_Sixstrainswithhigherextracellularcellulaseact

9、ivitywereseparatedandpurifiedfrom316colonies.StrainA1hadthehighestcellulaseactivitysotheinfluenceofdifferentcultureconsistsandconditionsonthreekindsofextracellularcellulaseactivitywerecompared.Astheconclusionthecellulase

10、activityreachedthehighestlevelontheconditionof2hasreactingtime.Htstarchmediumasseedmedium3dasseedagestarchascarbonsourcepeptoneasnitrogensource0.10%asconcentrationofM擴(kuò)andP04330CPH7.2100mL250mLasbottleholding.6dasculturep