由成年波爾山羊體細(xì)胞產(chǎn)生的克隆羊.pdf

1、揚(yáng)州大學(xué)碩士學(xué)位論文由成年波爾山羊體細(xì)胞產(chǎn)生的克隆羊姓名：唐慧林申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：臨床獸醫(yī)學(xué)指導(dǎo)教師：成勇20020401唐慧林由成年波爾山羊體細(xì)胞產(chǎn)生的克隆羊!Absl：ractFibroblastswereseparatedfromtheboer21。(aboutlg)eartipandculturedinvitrofordonorcellstoPll～P12，andMIIstageoocytesweregotfromdel詛

2、oftheChangjiangRiverthroughsuperovulationWhenthecellsconnllcnceabout80％，apartwasselectedforsynchronizationtoMstages：the銜ststep，treatwithThymidinefor16hours，thenrecoverculturefor5hours，thethirdstepwastreatit1rithNocodazol

3、efor78hours，thelastwas30minutesbeforetransferselectthecellswithdigestion；theotherwastreatedwithnothingandselectfornucleartransferThereconstructedembryoswhichwereproducedbyelectrofusingthedonorcellsintotherecipientcytopla

4、stswereculturedfor46hoursbeforeactivatedAfteractivated，embeddedtheseembryoswith1％agarosefortwotimes。thentransferdthemintotheligatedoviductofsynchronizedrecipientgoatResults：(1)Thedifferencebetweenthefusionrateandthedevel

5、opmentrateofreconstructedembryoswhichweretreatedwithThymidineandNocodazole(4057％、4102呦andwhichweretreatedwithnothing(4464％、4265％)werenotobvious：(2)ThereconstructedembryosofboergoatsomaticcellsandtheMIIstageenuclearoocyte

6、sweredeveloptoterm：(3)Thedonoreellswhichwereusedfortransferwereculturedinvitl“OtoP11～PJ2，thesesuggestafterlongtimeculture如riLroboergoaxtcellsalsohavetotipotentpotentialofdevelopment：(4)Intheory。Thedonorcellswhichweretrea

7、tedwithchemistryregentatMstageusedtonucleartransfercangetclonedgoat(5)Fromthephenotype，suggestthattermisaclonedboergoat：(6)ThealalyseofmicrosatelliteDNAfurtherprovedthattermisaclonedboergoatKeywords：nucleartransfer，hybri