同工酶和RAPD標(biāo)記在金針菇菌株鑒定和遺傳多樣性研究中的應(yīng)用.pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文同工酶和RAPD標(biāo)記在金針菇菌株鑒定和遺傳多樣性研究中的應(yīng)用姓名：黃晨陽申請學(xué)位級別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：黃毅2002.5.1塑壟壘查苧蘭翌主蘭竺壘蘭————————_三一AbstractInordertostudythegeneticdiversityofthe32testedstrains，randomamplifiedpolymorphicDNA(RAPD)andpolyacrylamidegelel

2、ectrophoresis(PAGE)wasappliedinthispaperEsterase(EST)isozyme，ployphenoloxidase(PPO)isozymeandperoxidase(PO)isozymewerestudiedwithPAGEThegeneticdiversitywasanalyzedintegratedwiththecultivatedcharacterThat，theapplicationof

3、RAPDmarkersinstrainsidentificationandtheferrecastoftheeminentstrains，alsowasdiscussedinthepaperItshowsthat：1Combinedwiththecultivatedcharacterandtheoriginbackground，therewasevidentdifferencebetweentheresultoftheclusterin

4、ganalysisofisozymeandthemorphologicalclassifyTheresultoftheclusteringanalysisofRAPDisrelativelyexactInthetestedstrains，theyellowstrainsweremorediversityInmorphologyaccordingtothecolorofthecapoffruitbodyFlammulinavelutipe

5、sweredividedintotwogroups，theyellowstrainsandthewhitestrainsButtheresultoftheclusteringanalysisofRAPDshowsthat，theyellowstrainsandthewhitestrainsdidnotdividedintotwogroups，andthereisinherentrelationinthemTheheredityvaria

6、tionisnotobvious2That，whetherthecapofthefruitbodyisapttoopenornot，influencetheeconomicvalueofthefruitbodyPCRWaSdonewiththe$22primerWefoundaDNAsequenceintheproductionofitThesequencewascorrelatedwiththestrainsapttoopenItis

7、about11kb3TheresultofRAPDalsoshowsthat，PCRWaSdonewiththeeightselectedprimersTherewere廿1irtythreepolymorphicsequencesintotalbywhichwecanidentifythisstrainForexample，intheresultofthe18thandthefirststrain，whichPCRwiththeS7a