偶氮類色素半抗原與人工抗原的合成及應用研究.pdf

1、南開大學碩士學位論文偶氮類色素半抗原與人工抗原的合成及應用研究姓名：劉亭亭申請學位級別：碩士專業(yè)：有機化學指導教師：王文虎201205AbstractAbstractEdibledyesarewidelyusedfoodadditivesinfoodindustryforimprovingfoodcolorandlusterThefrequentlyusedEdibledyesaresyntheticedibledyes，andmost

2、oftheseareazodyes，whichbystudiedinducehumanlesions，arepotentialcarcinogenicInrecentyears，thefactofazo—ayesusedlawlesslyinfoodhasaseriousimpactonfoodsecurityAtpresent，thestandardmethodtodetectazodyesinfoodisinstrumentsmet

3、hod，becauseithasthesuperiorityofhighaccuracyandlowdetectionlimitAtthesametime，thismethodhasalotofdisadvantages，eg，expensivecost，time—consuming，etcTheenzyme—linkedimmunosorbentassay(ELISA)methodisasensitive，rapid，simpleop

4、eration，cheepmethod，SOithaswideapplicationprospectUptonowonlyafewofazodyeswhichcontainactivegroups(eg，COOH，NH2)canbedetectedbyELISAmethodItisindispensibletostudyandestablishnewELISAmethodsofazo—dyesThekeytoestablishnewEL

5、ISAmethodisthesynthesisofexcellenthaptensandrelatedartificialantigensInthisdissertationweestablishednewroutestosynthesizehaptensofazodyesWesuccessfullysynthesizedfivehaptensofSunsetYellow(18、21(ac)、24)andfivehaptensofAll

6、ureRed(29、32(a—c)、35)ThroughacylationofhydroxylgroupofazodyeswealsosynthesizedthehaptensofsudanredI，acidorangeII，brilliantyellowsunsetyellowandallurared(60a60e)，respectivelyTheresultsseRleagoodfoundationfortherelatedELIS

7、AmethodThestructuresofallthenewfiftyonecompoundswereconfirmedby1HNMRandHRMSInaddition，thecrystalstructureofcompound47ewasconfirmedbyXraysinglecrystaldiffractiondeterminationKeywords：foodsecurityazo—dye，ELISAmethod，hapten