低溫保護劑溶液降溫結晶過程的實驗研究.pdf

1、中國科學技術大學碩士學位論文低溫保護劑溶液降溫結晶過程的實驗研究姓名：張永鋒申請學位級別：碩士專業(yè)：工程熱物理指導教師：何立群20040501AbstractInCryopreservation，thecrystallizationofCryopreservationagent(CPA)solutionandresultingintracellulariceformationarethemainreasonsthatcausedamag

2、etothecellstobepreserved，AstheenvironmentcellsareIocatedtheevolutionofCPAsolutionintheprocessofcoolingplaysavitalrolethataffectsthesurvivalrateofcellsInthispapertheconventionalLaserWollastonPrismShearingInterferometerwas

3、modifiedandcombinedwithasemiconductcoolingunitUsingthissystemweinvestigatedandrecordedthewholeprocessoftheformationandevolutionoficecrystalin5％KCLsolutionDifferentevolutionprocesswasobservedunderdifferentcoolingrate，Thei

4、nformationoftemperatureandconcentrationdistributioninthecoolingprocesscanbeobtainedafterthepostprocessingofinterferogramsAlsowecanstudytheaffectionofcoolingrateandconcentrationtotheprocessofcrystallizationconvenientlythr

5、oughserialexperimentsThisworkwillprovidesolidexperimentalgroundworkforcryopreservationOntheotherhand，westudiedthe2DiceformationinsolutionbetweentwocoverslipsindifferentcoolingproceduresTheobservationwasconductedunderacry

6、omicroscope，andthedynamicaliceformationinsampleisrecordedbyavideocamerainfreezingprocessWefindthatinthecaseofhomogeneousnucleation，theicegrowsveryquicklyandthetextureisveryfineInthecaseofinhomogeneousnucleation，theicegro

7、wsthroughthreedifferentstagesintermsoftheshapeofborderlines，thecircle，hexagonalandtreelikestructureThethirdisregardedmostharmfultocellmembraneforitssharpendsDuetoitswideexistenceinfreezingcellsuspension，theinhomogeneousn