誘導(dǎo)子對(duì)西洋參發(fā)狀根的影響.pdf

1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文誘導(dǎo)子對(duì)西洋參發(fā)狀根的影響姓名：靳浩淼申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物生物技術(shù)指導(dǎo)教師：王義20080601ResearchofelicitorinAmericanginsengrootAbstractInthispapertheAmericanginsengrootforthematerial，inthecallusinductiontheoptimizationofcultureconditions，induce

2、dbythesonofAmericanginsengrootswiththeimpactofsuchareaswerestudiedKeyfmdingsareasfollows：neeffectsofdifferentexplants，pretrainingtime，atotalculturetime，theconcentrationofbacteriaandbacilliASaddtotherootofAmericanginsengi

3、nducedeffectsThroughexperimentsthat：R1601inducfionofAgrobacterium12weeksAgeofAmericanginsengleaves，stemsandleaveswithDavidStem，Davidleavestostemswiththerootofthehighestratesofinduction，pretraining48ll，afterinfectionTheex

4、plantsculturedinatotalof48hwillgetbetterresultsActivationoftheprocessofadding100Hreel／LAShairyrootsCallincreasetherateofinductionSuspensionofopticaldensitycontrolOD600=06，with20raintimeforoptimalconditionsIntheoptimizati

5、onofcultureconditions，respectivelywithfourkindsofliquidmediumMS，improvedW1lite，SJlandB5cultureofAmcricanginsengrootsSJ1liquidmostconducivetothegrowthofroots，ongrowthinmultiplesofupto313timesthatoftheliquidundertheconditi

6、onsofthetrainingMS17timesThetotalaccumulationofsaponinWhiteimprovedtothebesteffect(810％)TherootoftheintegratedgrowthandtheaccumulationofsaponinMSmediummostbeneficialSucrosebestinterestsoftheAmericanGinsengrootgrowthandal

7、sothemostconducivetotheaccumulationofsaponinThemostappropriatcofAmericanginsengrootgrowthandtotalsaponinofthepHvalueis58InductionoftheAmericanGinsengroot，theimpact，inducedbytheadditionofonehandcanenhantethemetabolicactiv

8、ityofcertainenzymesfollowedbyfurtherenhancingtheaccumulationofmetabelitesofHealthontheotherhandtheadditionoflcadinducedeellactivitydecreasedandeveneelldeathWhenindueedbyexcessiveconcentrationeellactivitydecreasedsynthesi

9、sofsecondarymetabolitesoftheimpactofincreasedgradually，overtheinductionofsynthesisofsecondarymetabolitesinthepromotionandthereforeinhibittheaccumulationofsecondarymetabolites111ispaperstudiesthesalicylicacidmethyljasmona

10、tetwoofthebestadd—inducedconcentration，timeandtheroleofthetwoinducedsynergiesEventuallycometotheconclusion：thebestconcentrationsofsalicylicacidforthe06mg／L，thetimefortheroleof144hAddthebestofsalicylicacidconcenWationandt

11、heroleandeffectsoftimeandMiaoZhiqi，wheportedthattheDeJiangLi，wheported血atWenchiLanYuLongwhoreportedtllebasiclineMostscholarsbehevethattheconcentrationsofsalicylicacidshouldnotexceed10mg／L，whentheacidconcentrationhigherth

12、an10mg／L，isnotconducivetoplantgrowthandtheaccumulationofsecondarymetabolitesAddmethyljasmonatethebestconcentrationfor10umol／L，thetimefortheroleof192h240hExperimentprovedthatmethyljasmonatecaninducetheAmericanginsengroots

13、econdarymetabolitesintheaccumulationofginsenosideMethyljasmonateoftheplanttopromotethisroleandthebestconcentrationofHunan，HubeiandtheSovietUnion，MeiXingguo，whoreportedthatChunRongZhang，LiLing’sreport，LiangXuSheng，XiaoFen

14、gXue，F(xiàn)uChunxiang，etcThereportswereconsistentwiththebasicMostofthescholarsreportedwhentheconcentrationofmethyljasmonatethan20umol|LwhenthecellswillhaveanegmiveeffectTwoinductionofthebestsynergyofsalicylicacidratioof04mg|L