膽管癌患者膽汁中端粒酶逆轉(zhuǎn)錄酶和Bcl-2基因的檢測及意義.pdf

1、南昌大學(xué)醫(yī)學(xué)院碩士學(xué)位論文膽管癌患者膽汁中端粒酶逆轉(zhuǎn)錄酶和Bcl2基因的檢測及意義姓名：路選申請學(xué)位級別：碩士專業(yè)：外科學(xué)（普外）指導(dǎo)教師：蔡軍20070520ABSTRACTABSTRACTObjective：1Tostudytheexpressionoftelomerasereversetranscriptase(hTERT)andBcl2inbileofpatientswithcholangiocarcinomaandtherel

2、ationshipbetweenthem2TodiscussthediagnosticvalueofthedeterminationoftheexpressionofhTERTandBcl2inbileforcholangiocarcinomaMethods：1Thecontrolgroup(20bilesamplesofpatientswithcholedocholith)Thetrialgroup(20bilesamplesofpa

3、tientswithcholangiocarcinoma)2ExpressionofhTERTandBcl2inbilewereconfirmedbyreversetranscfiptasepolymerasechainreaction(RTPCR)Results：1hTERTwereexpressedin14outofthe20bilesamplesofpatientswithcholan舀ocarcinoma(14／20)，butn

4、oexpressionwasfoundin20bilesamplesofpatientswithcholedocholith，whichwassignificantlydifferentbetweenthetwogroups(p005)；2Bcl2wereexpressedin11outofthe20bilesamplesofpatientswithcholangiocarcinoma(11／20)，butinonly1outofthe

5、20bilesamplesofpatientswithcholedocholith(1／20)，whichwassignificantlydifferentbetweenthetwogroups(p005)；3in10patientswithcholangiocarcinomasurgeryhTERTwereexpressedin6outof6bilesamplesofpatientswithmetastaticcholan舀ocarc

6、inoma(6／6)，whichwassignificantlyhigherthanthatofpatientswithnonmetastaticcholangiocarcinoma(1／4)(P005)In10patientswithcholangiocarcinomasurgeryBcl一2wereexpressedin4outof4bilesamplesofpatientswithhighlyormoderatelydiffere

7、ntiatedcholangiocarcinoma(4／4)，Bcl2wereexpressedin2outof6bilesamplesofpatientswithpoorlydifferentiatedcholangiocarcinoma(2／6)，therenosignificantdifferencebetweenthem4TheexpressionofhTERTandBcl一2inbileofpatientswithcholan

8、giocarcinomahadnocorrelationConclusion：1TheexpressionofhTERTandBcl2inbileofpatientswithcholan舀ocarcinomaweresignificantlyhigherthanthoseofpatientswithcholedocholith(p005)2TheexpressionofhTERTandBci2inbileofpatientswithch