磁性阿霉素納米脂質體靶向治療裸鼠大腸癌的實驗研究.pdf

1、復旦大學博士學位論文磁性阿霉素納米脂質體靶向治療裸鼠大腸癌的實驗研究姓名：周平紅申請學位級別：博士專業(yè)：外科學指導教師：秦新裕20030430ABSTRACTABsT扎iclTargetingTherapyofMagneticDoxorubicinNano—LiposomeinNudeMiceBearingColonCanceJ。Itiswellrecognizedthattraditionalsystemicchemotherapyh

2、asthelowtherapeuticindexandpoorpatient’scomplianceTargetingtherapyforsolidtumors，usingspecificcarrierstolocateanticancerdrugsselectivelytosolidtumorsunderspecificguidingmechanism，mayincreasethedrugconcentrationintherequi

3、redsitesSuchanapproachwoulddramaticallyincreasetheanticancerdrugs1therapeuticefficacy，reducethedrugs’toxicsideeffectsandincreasethepatient’ScomplianceThepresentstudyinvestigatedthemagneticnano—liposomedesignedtoactasdoxo

4、rubicin(DOX)carrier，whichcouldbeeffectivelydeliveredtoSOlidtumorsviaintravenousadministrationMagneticdoxorubicinnano—liposome(MDNL)waspreparedbythereverse—phaseevaporationmethodWestudiedtheresponseofMDNLtothemagnetitebot

5、houtofandwithinthebody，investigatedthebiologicaldistributionoftheMDNLinthenudemicebearingcoloncancerThetherapeuticeffectofMDNLtonudemicebearingcoloncancerwasalsointensivelystudiedTheresultsshowedthat：(1)Theparticlesizeof

6、MDNLwas230nm，theconcentrationofDOXinMDNLwas30099／ml，andtheentrapmentrateofDOXwasabout25％Differentlipidratio，druglipidratio，organicsolventandratioofwater／oilphasemayinfluencetheparticlesizeoMDNLandtheentrapmentrateofDOXTh

7、eleakagerateofDOXinMDNLwasverylowwithinthepreservative8hoursunder40Cafterpreparation(2)MDN[一hadagoodresponsetothemagnetitebothoutofandwithinthebodyAdministrationofMDNLundermagneticfieldcouldbeusedtodeliverDOXeffectivelyt