紫蘇光周期反應(yīng)與類(lèi)LFY基因cDNA的克隆.pdf

1、海南大學(xué)碩士學(xué)位論文紫蘇光周期反應(yīng)與類(lèi)LFY基因cDNA的克隆姓名：蘭樹(shù)斌申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：作物遺傳育種指導(dǎo)教師：李建國(guó)20080601紫蘇光周期反應(yīng)與類(lèi)即甲基因cDNA的克隆AbstractPemla(P洲婦夕甜幻scP珊L)isatypicalshortdayplantPhotoperiodinnuences恤gr0、砒anddeVelopmentofP鰣lla，itisdecisivet0regulatetheeXpress

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8、agelaSted34daysisnowerbuddi行erentiationofPe礎(chǔ)aiIlthemicroscopeA445bpcDNA∞脬lil【e)療agmentwaSclonedbyRTPCRandmmedpLFY，usingplantmaterialof“sphaSeSequencea11羽ysisshowedmatmecDNA觸舯enthadhomologyof90％晰tll凡DJ，67％晰tllB㈣9l％謝t11凡0嘣

9、2，67％晰tll￡n，，80％嘶tll兕DDesi弘ingmePCRp灑ersusing也ecDNA螄弘entsequenceofPerillaA308bpcDNA觸鯽entwaSclonedby37RACEAnewcDNA觸gmentsequenceof、^，asmadeupoftllistw0sequencesTheresultofBlaS欽sho、張dmatmeSplicedfhgmentsharedaKghde鏟eeofseq