膽固醇疏水改性葡聚糖-聚乳酸雜化囊泡的形成機(jī)理及藥物釋放研究.pdf

1、天津大學(xué)碩士學(xué)位論文膽固醇疏水改性葡聚糖聚乳酸雜化囊泡的形成機(jī)理及藥物釋放研究姓名：張志華申請學(xué)位級(jí)別：碩士專業(yè)：材料學(xué)（高分子）指導(dǎo)教師：原續(xù)波20060101ABSTRACTCholesterol3一hemisuccinylchloridewassynthesizedbycholesterolandsuccinicanhydrideinanhydrouspyridine，whichwascharacterizedbyinfrared

2、spectroscopycholesterolhydrophobicallymodifieddextran(DexCH)wasobtainedbyesterificationofdextrancholesterol3hemisuccinylchlorideusinganhydrousdimethylsulfoxide(DMSO)assolutionandterethylamineascatalyst，substituteddegreec

3、allbecontrolledbytheratiobetweendextranandcholesterol3hemisuccinate，theactualsubstituteddegreeWaSanalysizedby1HNMRspectraPoly(acrylicacid)andDexCHweredissolvedinDMSOincertainproportion，thendialysisagainstdeionizedwater，P

4、LA／DexCHvesicleWaspreparedFromtheexperiment，itindicatedthatthevesicleformationWashighlydependentontheproportionofPLAandDexCH，themolecularweightofPLAWas510ag／molanddextranwas4X104g／mol，substituteddegreeWas8％，whentheratioo

5、fPLAandDex—CHWasintherangeof1：7and2，vesiclestructurewasformed，whichCanbeobservedbyTransmissionElectronicMicroscopy(TEM)GadoliniumChloride(GaCl3)，contrastmedium，couldincreasethevesiclecontrasts，intheconditionofhighermagni

6、ficationtime，thevesiclelayerWassandwichstructure；thefluorescencespectratechnologycanindicatetheexistenceofhydrophobicmicrodomainsIn1HNMRspectra，thesignalofPLAandcholesterolmoleculesweresignificantlybroadeningandlossinres

7、olutiondemonstratedthatthevesicleformationwascontrolledbyhydrophobiccomplexation，throughthatinteraction，PLAwasjustlikealongchainmoleculegraftedontodextranIndifferentproportionofPLAandDexCH，changethemolecularweightofPLA，v

8、esiclecouldformedinthemiddleproportion，andtherewerenelationshiptoPLAmolecularweight；thesubstituteddegreewere4％，8％，17％，26％，respectively，andchangethesubstituteddegree，vesiclestructurecouldbeformed，vesiclestructurecouldnotb

9、eformedinhigherorlowersubstituteddegree；dextranwithdifferentmolecularweight(sx104g／mol，105g／mol，3105g／mol，5X105g／m01)，vesiclestructurecouldbeformed；vesiclestabilitycouldbeinvestigatedbyzetapotentialandturbidityThehydroph