可用于中草藥有效成分提取的纖維素酶的固定化研究.pdf

1、重慶大學碩士學位論文可用于中草藥有效成分提取的纖維素酶的固定化研究姓名：周熙云申請學位級別：碩士專業(yè)：藥物化學指導教師：王遠亮20091202英文摘要ABSTRACTCellulaSesaree叼rIIlest脅hydrolyzethep一(1—4)linkagesincellulose111ey盯eproduced嬲amulticomponentenzymesystemcomprisedusuallyofthreeenz)，mes：e

2、ndocellulaSes，cellobiohydrolaLses，鋤dp—glucosidasesTheseenzymesfomacellulolyticsystem鋤dhydrolyzeinsolublecelluloseinaVe巧emcientandsyne曬Sticwly，socellulaSes11a_veeno冊ousbiotedllll0109icalpotemialinVariousindustries，includi

3、ngthephaImaceutical，food，鋤dchemicalengineering，etcWhatisnotewormyspecially，aScellulaLsescalldecomposemecanlpaCtstnIctureofVegetalceU、Ⅳalla11dresultiIlt11edissolutionofe毹ctiveherbalingredients，maIly舭shaChievememsinextmcti

4、ngherbalingrediemsbycellulaSehaVebeenmadeinrecentyearsNeVertheless，tllepractical印plicationofcellulaSesisstillfairly1imitedThemainreasonist11ehighcostofenzymebecausefkecellulaSesaresusceptibletoiIlaCtiVationaLs、veUasdi銜cu

5、lttoisolation，purificationandrecyclesⅥlriousmethodslIaVebeenreportedforimmobilizationofceUulasesAcr)rlicpolylnerswIlichconlainactiVe毋oupsaresui訕leforimmobilizationenzymesInmysmdy，polymethacr)，licacidgelandpoly(ac巧licacid

6、—co—hydroxyemylmethacrylate)ma印eticmicrospheres、ⅣeresynthesizedCellulases、Verei刪mobilizedontothesecarriersThee虢ctsofamountofcellulases，di腩rentbu行erpH，immobilizationtiIIle，concentlationofactivatingagentonme鋤ouIltofenzymel

7、oading，enZymeactiVi壩specificandretainedactiV時ofimmobilizedemEylnewereiIⅣestigatedSomecharacteristicsofimmobiliZed砒ld丘eecellulaSes，suCh嬲optimumtemperature，optim啪pH，Michaelisconst趾t，methemlal，operationalarldstorages訕ilitie

8、swereinVeStedandcomparedThem曩incontentsareincludedasfollows：1Cmss—lin玉【ingpolymethacrylicacidgel、^，aSsyI】血esizedCellulaseswereimmobilizedontot11iscarrierbyadsorption，coValentcouplingaIldcrosslilll(ing，mldtheimmobilizatio

9、ncorlditionsareoptimizedThe鋤ountofenzymesloadingofimmobilizedenzymesbyadsorption，coValentcouplingaIldcrosslinkingis19，14and1Om∥g，andt11eactiVit)，retentionis456％，308％a11d228％，respectiVely2SomecharaCteriSticsofcellulaSesim

10、mobilizedonPMAAaremVestigated111eoptim啪temperatureofimmobilizedcellulaSesrisesby1O℃TheoptimumpHofimmobilizedcellulasesbyadso叩tion2uldcoValentcouplingis56and66respectivelyImmobilizedcellulaSesbycrosslinkinghaSrelatiVely址g