石菖蒲根莖提取物β-細辛醚對谷蠹的生物活性測定及作用機理研究.pdf

1、華中農(nóng)業(yè)大學碩士學位論文石菖蒲根莖提取物β細辛醚對谷蠹的生物活性測定及作用機理研究姓名：宋旭紅申請學位級別：碩士專業(yè)：農(nóng)業(yè)昆蟲與害蟲防治指導教師：楊長舉20070601華中農(nóng)業(yè)大學2007屆碩士學位論文Abstractn地contactactionrepellencyandfumigationactionof爭asaronederivedfromtherhizomeofAcorusgramineusagahlstRhizoperthad

2、ominica①ab)adultusingdrugfilmmethoddrugramselectinginfilterpaperandpaperfumigationinOassjarswerereportedinthis華驢札TheresultshowedthatBas鋤咖hadbetterbioactivitiestdominicaadultat72hposttreatmentthecorrectedmortalityat1592耀r

3、／cI∥WaS100％，andthemortailtyregressionline鋤dLDs0ofcontactactionw圈teY=2893852530xand3182Il咖I∥correspondingly11“repellencelevelof1573，7865，3933，1966pg／cmZwcleallmorethanⅣn峙correctedmortalitiesat10000，50002500，1250625肛眈werea

4、llmorethan8000％at120hposttreatmentbyfumigattngThcfumigationregressionlineandLCsowereY音317275xand462uL兒Inintegratedtoxicitytest定dominicaadultappearedpoisomlsymptomjustlikelosingselfdeterminationmovementabilityandunrespons

5、iveat48hposttreatmentThemortalityof足dominicaadultswaSeptl越lcedquicklyafter72htreatedTheintegratedregressionlineandLDsowereY=1016767xand9449mg／Kg爭asaronewasmixedwithwheattostudytheinhibitionactionagainst殳dominicaprogenyTh

6、eresultshowedthatithadbettercontroleffectto兄dominicaAt20000mgn(gand15000mg／Kgtreatmentsthecorrectedmortalitieswere100。00％and9667％respectivelyat7dposttreatmentTheinhibitionratetoprogenywere10000％and9884％respectivelyat60dp

7、osttreatmentThedalnageratestowheatwere000％and133％，whichweremarkedlylowerthanthedamagerate(5OO％)ofMalathiontreatmentSeveralkindsofimportantalzyme$in足dominicaadultw啪studieda船rU℃atadwithB—asaroneTheresultindicatedthatcnzym囂

8、activitiesrepresentedstrongertimeeffectionAcetylcholinesterase(AChE)andGlmathioneStransferase(OSTs)aativitiveawereinhibitedbyBasaronewiththeprolonglingofexposuretnne砒9449mg／ggdosageWhereas，theactivitiesofCaxboxylesterase

9、(CarE)andEsteraseisoenzyme(ESTisoenzyme)woreinducedby[1ssaroneatthesarfledosageWhen見dominicaadultwaStreatedwith13asaroneat675，800，1000，1333，2∞Omgngdosages，theacfivitesofAchEandGSTswereinducedat675mg／l(gpasaroneandinhibit