我國玉米大斑病病原菌變異研究.pdf

1、Y渺m■：一＆』L、。一西弛農(nóng)林針杖大學2006屆攻讀項士學位研究生學位(畢業(yè))論文我國玉米大斑病病原菌變異研究學科專業(yè)擅塑瘟型堂研究方向擅塑遁置堡盒造堡研究生王垂蓮指導教師當直熬握王鯉盟亞宜雖RACEVARIATIoNoFEXS!ERD月刃硎TURCICUMCAUSALAGENToFNORTHERNLEAFBLIGHTINCHINAABSTRACTNorthernCOHIleafblightorTurcicumleafblightis

2、themostseriousdiseaseinthemaizegrowingareasallovertheworldn地OCC411TClltTA：andepidemicsofthisdiseasearcaffectedbythecompositionandvariationofExserohilumturcicumpopulationThispaperfocusesonidentifmginpathogenicvariationtod

3、ifferentHtresistancegeneandthepopulationstructureofthepathogenbasedonAFLP丑蝣resultsareofsignificanetothercsistantbr∞dingaswellastocontrolvariationofphysiologicalr；K器ofNortherncornleafblightOnehundredisolatesofExaerohihunt

4、urc／cum，collectedfrom23provincesorcitiesin2005and2006wcreidentifiedinpathogenicvariationtodifferentHtresistanceg廿圮inchamberneresultsshowedthatcompositionofthephysiologicalraceswascomplexinabove23provincesorcitiesTherewe∞

5、15differentIaeA囂inthe100isolatestestedRacel。o’Nshowedthehigherfrealucncyof21％18‰13‰followedby13，IN2，123，3N2N3，23，123N12，12N23Nwithfrequenciesof2％～8％Atthe鯽etimephysiological齜variationsintheNorthan濰archigherthanintheSouth卸

6、陀a&AnAFLPprotocolforanalymgExserohilumturcicumWasestablishedafterselectingtheconditionofreactsystems11塢sixisolatescollectedfromdifferentprovincesWCI“eamplified稍Ih“11竹，“l(fā)2”，‘‘22’：‘‘23”’‘‘33”primerscomplicationsTwentysixpa

7、irsofprimma∞polymorphicamplificationinthestrainsofExaerohilumturcicumElevenpairsofprimersⅥ，cmusedforamplifying90isolatesinaboveacrasTheresultsshowedthattherewerenodirectcorrelationamongphysiologicalnIces、geneticdiversity