雞內源性類J亞群禽白血病病毒gp85基因的克隆及表達.pdf

1、內蒙古農業(yè)大學碩士學位論文雞內源性類J亞群禽白血病病毒gp85基因的克隆及表達姓名：童淑梅申請學位級別：碩士專業(yè)：基礎獸醫(yī)學指導教師：趙振華楊玉瑩20070501CloningandExpressionofChickenEndogenousSubgroupJAvianLeukosisgp85一likeGene^bstraol：AvianleukosisVI‘RUSsubgroupJ㈣J)wasfirstisolatedin1989and

2、identifiedasanewsubgroupofavianleukosisvirusmenvgeneofALvJisveryunique。hasverylowhomologytOotherALVsubgroups，howeverexhibits97％identitytOEAVHPsequfncewhichisexistedinthenormalchickensgenomeALVJmostcausesmyeloidleukosis，w

3、hileotherexogenous^￡飴mainlyinducelymphoidleukosis力培previousresearchdatashowedthatenVgeneiscloselyrelatedtotheALVoncogenicityEnvgenecodevirusenvelopeglycoprotein，includingSurfaceglycoproteinUnit(gp85genecode)andtransmembr

4、aneprotein(gp37genecode)PrimerscorrespondingtothenucleotidesequencesflankingtheJsubgroupavianleu—cosisvlrus(ALVJ)gP85genewereusedtoamplifytheintactendogenousgp85一likege—nefromuninfectedSPFembryo、Meatchickembryo、Liangfeng

5、huachickembryoandNativechickThefourdifferentvarietyendogenousALVJgp85likeelementsdemons廿ated952％～98。6％identicaltoDFIcellLinereported，949％～976％identicaitostrain：HPRS103AIVJ885％～907％identicaltostrainIMC10200ALvJForresearch

6、indepthendogenousALVJ99851ikeexpresswhatparticipanta醴on也】ringinfectingm”J，itpro—videsafavorablefoundation一msystemOfBactoBacwastriumphantlyconstructed，andexpressedALVJgp851ikeproteinALVJgp85一likeproteinexpressedwasidentif

7、iedbymono—clonalantibodyJE9tOALVJgp85protein，byIFAandWesternblotdetecfingThemolecularweightofALVJgp851ikeproteinwas35KD船themolecularweightofALVJgp85protein(36KD)notglycosylatedTheresearchrevealstheactivestateofALMJgp851i

8、keinnormalconditionandtheimmunologycolmnortllessanddiscrepancywithexogenousvirusgp85proteinexpressed吼erecombinedbaculovirusconstructedmaybemultipledlargelyandCanobtainlargenumbersofenvelopeproteinwithbiologyactivitywhich

9、canbethedetectionantigenofELISAinsteadofALVJgp85proteininthefuture，designedtodetecttheinfectionofALVJvirusTheresearchiSverysignificanttorealizetheevolutionofretrovirusandthemechanismofitsinfecti∞Keywords：Avianleukosisvir