榛子種質(zhì)資源多樣性的RAPD分析.pdf

1、遼寧師范大學(xué)碩士學(xué)位論文榛子種質(zhì)資源多樣性的RAPD分析姓名：張希踔申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：馮斌20070525摘要AbstractHazel(corylus)isanimportanthorticulturalplantbelongingtoCorylusBetulaceaeDuringthecourseofevolutionandbreedingmanyvarietiesweredevelopedThestudy

2、ongeneticdiversityofcoryluswouldbenefitthegermcollection，identification，geneticnovationandeffectiveutilizationofcorylusMolecularmarkerscandetectthegeneticdiversityatDNAlevelandbecomeapowerfultooltostudygeneticdiversityIn

3、ourresearch，thetechniqueofseparationandpurificationofgenomeDNAofCorylusWaSoptimized，andrandomamplifiedpolymorphicDNA(RAPD)reactionsystemforanalysisofcorylusDNAwasestablishedRAPDanalysiswascarriedouttomeasurethegeneticdiv

4、ersityof48accessionsofCorylus(include4species)，whichwerecollectedfromEurope，AmericaandNorthernChinaTwentysixpolymorphicprimerswerescreenedoutof150randomprimersand142bandsweredistinguishableandreproducibleOfthem，130bands(

5、915％1werepolymorphicThe48accessionsweredividedinto5groupsaccordingtotheclusteranalysisCorylusavelanaandCheterophyllabelongtothefirstgroupThesecondgroupincludes30varietiesthatareallhybridCorylusexceptthe39(T81一鰳，whichisac

6、heterophyllaThethirdgroupincludes5varieties：22(G80—0452)，29(A81—3)，30(K83—1)，34(A一84206)，45(K一83—5)thatareallCheterophyllaThefourthgroupincludes10varieties：1(43)，2(Royal，4(50)，12(TDGL)，3(TDG)，5(Barcelona)，10(MONTONBELLO)

7、，7(GRlFOLLl，8(GIAnt)，9(SIVIRI)AndthevarietiesinthelastgroupareallhybridCo咖sThemaximalandminimumsimilaritycorrelationsbetween48CorylusvarietiesareO，88and046respectivelyThearveagesimilaritycorrelationsofCheterophyllaandCav

8、eHanaare075and074respectivelyHowever，thesimilaritycorrelationsbetweenCheterophyllavarietiesandCavellanavarietiesareallcloseto053OurresultsareconsistentwiththetraditionaltaxonomyandprovidemorevaluableinformationforCorylus