DNA芯片動力學(xué)初探及應(yīng)用cDNA芯片研究前列腺癌表達(dá)譜.pdf

1、Y770313後旦大擎碩士學(xué)位論文學(xué)校代碼：10246學(xué)弓：022023038DNA芯片動力學(xué)初探及應(yīng)用eDNA芯片研究前列腺癌表達(dá)譜院系：專業(yè)：姓名：指導(dǎo)教師：完成日期：遺傳所遺傳學(xué)王玉李瑤教授2005年5月6日復(fù)旦大學(xué)碩士學(xué)位論文AbstractDNAmicroarraycanbeusedtomeasureDNAandRNAvariationinapplicationsincludinggeneexpressionprofiling

2、，comparativegenomiCS，andgenotypingTheanalysiSofgeneexpressionprofileiSodeofthemostcommonapplicationsofDNAmicroarrays。Expressi011microarraysareoftenconstructedbyimmobilizingprobesofeDNAandoligonucleotideontwo—dimensionalm

3、odifiedglassSlidesIntheutilityofcurrentmicroarrayformats，thehybridizationefficiencyresultsfromthecombinationofmanyfactorsThedynamicbehavioriSakeyaspectinhybridizationefficiencyandmakesanimportantcontributiontothefinalSig

4、nalintensityofDNAmicroarrayAtheoreticaldynamickineticmodelwasderived，andaseriesofexperimentscarriedoutUSinglOW—densitymicroarraysinvariousconcentrationsofspottingprobe([P])andlabellagtarget([T])Ithasbeenshownthattargetan

5、dprobedeterminedthesignalintensitytogetherAtacertainrangeofDNAconcentrationthesignalintensitywasinproportionofspotting[P]AtthehigherDNAconcentrationtherewasadecreasetendencyinhybridizationsignalintensity，especiallyincDNh

6、microarraySinceDNAmicroarraywasconstructedonthesolidsurface，sterichindrancethatinducedbythesolidsurfaceandthehigh[P]decreasedtheprobeimmobilizationefficiency，leadingtothedecreaseoftheinwnobilizedprobedensityThedecreasedh

7、ybridizationefficiencycausedthecompressioninsignalintensitywhenthetargetincreasedaswel1Nevertheless，theintensityratiovalueofCy5toCy3wasnotcompressedwithinamicroarrayinthetwo—colorsystemTheratioofCyS／Cy3isonlydeterminedby

8、theratiooftwotargetsandindependentofthedensityandthetypesofprobeIntheapplicationresearchofDNAmicroarray，weusedeDNAmicroarraystoprofilechangesingeneexpressionofprostatecanceronagenomicscaleGeneexpressionprofilesof7androge