農(nóng)桿菌介導(dǎo)的雙價抗蟲基因轉(zhuǎn)入大豆的研究.pdf

1、東北師范大學(xué)碩士學(xué)位論文農(nóng)桿菌介導(dǎo)的雙價抗蟲基因轉(zhuǎn)入大豆的研究姓名：高聳巍申請學(xué)位級別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：石德成趙桂蘭20070601東北師范大學(xué)碩士畢業(yè)論文摘要AbstractSoybeanswereinjuredseriouslybypestsduringtheperiodofdurationwhichresultedingreatlossofproductionofsoybeanThewidelyuseofchemical

2、insecticidenotonlyenhancedpesticideresistance，butalsodestroyedtheusefulinsectsandotherecologicalfloraAlso，itpollutedenvironmentseriouslyandincreasedthecostpriceofproduce，anditbrokethebalanceofecologyBecausetheproduceofbr

3、eedingWaStoolong，andthegeneticresourcewerelimitedandthemechanismofpestresistancewasunknownaswellasthedevelopmentofnewpestsresultedintheinstabilityofresistancetheroutinemethodofbreedingandplantingtechnologywerelimitedinso

4、meextenLwhichproveeffectswerelittleAsaresultithasbeenpaidcloseattentionthatusingplantgeneticengineeringtechnologytoenhallcethepestresistanceThepGBll21S4ABCcarriedtwoinsecticidalgenes，CpTIandGFMCrylAB上weresuccessfullytran

5、sferredinto2soybearl(GlycineMax)cultivars，“Jilin20”and“Jilin27”v／aAgrobacteriumtumefaciensmediatedtransformationImmaturesoybeancotyledonswereused嬲recipientAftercocultivationofSomaticcmbryogeniccallicinducedfromtheimmatur

6、esoybcancotyledonasrecipientmaterialswithAtumefaciensonscreeningmedium，somaticembryosandregeneratefertiletransgenic#antswereobtainedTransgenicsoybeanplantsharboringtwoinsecticidalgeneswereconlLrmedbyPCRandGUStestingKeywo