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1、UniversityCode：10225RegisterCode：09011DissertationfortheDegreeofMasterEffectofDroughtStressonTotalalkaloidsandflavonoidContentinLeonurusheterophyllusCandidate：Supervisor：AssociateSupervisor：AcademicDegreeAppliedfor：Speci

2、ality：DateofOralExamination：University：LiLinProfYanXiufengMasterCellBiologyMay2009NortheastForestryUniversityAbstractLeonurusheterophyllus，aherbaceousannualorbiyearlyoftheLamiaceaefamilyistraditionalChinesemedicineusedfo

3、rgynecologicalmedicinewiththebloodhemostasisandrelievingmenstrualpainandSOonThetotalalkaloidandflavonoidcontentsaredifferentasresultofLheterophyllusseasongrowth，ecologicaltype，plantmorphologyaswellasenvironmentalconditio

4、nsThisexperimentstudiedthedynamicchangeswhichthetotalalkaloidandflavonoidcontentsofleaves，stemsandrootsinLheterophyllusoccurredtoduringthesoildroughtandPEGstressItlaysthefoundationforLheterophyllusreasonableandeffectiveu

5、seofresourcesThemajorfindingsofthisstudyareasfollows：ItWascloselyrelatedthatdroughtstresstimeandthetotalalkaloidcontentofleaves，stemsandrootsinLheterophyllusThedifferentpartscorrespondingtothedifferenceduringdroughtstres

6、sWithincreasinginthenumberofthesoilnaturaldroughtdays，thetotalalkaloidcontentofleavesinLheterophylluswasagradualincreaseandthetotalalkaloidcontentofthesteminLheterophyllustendedtoslowafterthefirst5d，whilethatinrootshowss

7、ingle—peakcurveWithgradualincreaseintheconcentrationofPEGwhichusedtosimulatedroughtstress，itWasfoundthattheleavesandstemstotalalkaloidcontentsstarttoincreasewhenhefirst3dofdroughtstressinLheterophyllus；theroottotalalkalo

8、idscontentstartedtoincreaseinthedrypreprocessingThehighconcentrationofPEGusedtodealwith4h，theLheterophyllusleafstemandrootdidnotoccurintheaccumulationoftotalalkaloids，withnosignificantdifferenceincontr01Therefore，thatash

9、ortperiodoftimetodealwiththedroughtdidnotmakethetotalalkaloidcontentinLheteroph)，llusincrease；ThetotalalkaloidcontentofleavesandstemsinLheterophyllusstartedtoincreaseuntildealedwithatleast3daysComparedtoleavesandstems，it

10、wasealier也atthetimeofthetotalalkaloidscontentinrootstartingtoItisdirectlyrelatedbetweendroughtstresstimeandtotalflavonoidcontentofleafstemandrootinLheterophyllusTheflavonoidscontentofleaves，stemsandrootsofinLheterophyllu

11、sstartedtoincreaseinthethirddayduringsoildroughtWithgradualincreaseintheconcentrationofPEGwhichusedtosimulatedroughtstress，theflavonoidsfromtheleavesandstemsinLheterophyllusstartedtoincreaseat3daybutrootsisearlierAferthe

12、highconcentrationofPEGstressLheterophyllus，itwasfoundthattheflavonoidscontentinLheterophyllusreduced，butonlythestempartsignificantlyreducedTherefore，short—termdroughtstressdidnotmakeLheterophyllusincreasetheflavonoidscon

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