肝細(xì)胞移植術(shù)作用機(jī)制的實(shí)驗(yàn)研究.pdf

1、東南大學(xué)碩士學(xué)位論文肝細(xì)胞移植術(shù)作用機(jī)制的實(shí)驗(yàn)研究姓名：甘向陽申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：影像醫(yī)學(xué)與核醫(yī)學(xué)指導(dǎo)教師：滕皋軍20050501英立摘要EXPERIMENTALSTUDY0NMECHANISM0FEFFECT0FHEPATOCYTETRANSPLAN丑氣T10NAbstractObjcctjVe：TbestaMjshandopcrimcntalmodel血ath印atoc”etranspIan住dion扛eatstheacutel

2、iverfaIluroofratsinducedbyD—Galactosamjne，pH)vcthchypothesisofanimponante豌ctu，hjchtheprollterati呷anddlflewnIiationofhop“covalceIlshaveexenedonthe1jverrcgcnerationa船rratliverhasbeenimpalredandexp。糟themechanismthath。pa眥yte

3、cransplantatjonreversesdlcacl】砣liverfanurecausedbyD—GaIactosanlineMethods：Hepatoc”eHansplantatIonwascar『iedouljn仃apemonealIyanertheacuteIjverfailurcofralshadbeeninduccdbyD—GalactosarnjneThee11tireexp。ri眥ntlnclud甜PanIandP

4、an兒InPanI，thcseratswereinjectedbyDGalacto鞠mille(195臥酌andimInediatelydividedrdndomlyintoGroupAandGro“pB48hourSaRermtoxlcation410。allogene【chepatocyteswerotran印lantedintrap。ritoneaIIyinIoev。ryraI“10ro“pA，whlle10mlofnomlals

5、alinewereInJectedjntr印erltoneallyascontrolinGroupBThenthesurvjvaIra船fortwoweekswasassessedInPanII，aftcrtheljver血nc【ionindex，liverhistology如dAFPofthenomlalratswercobse“ed，tlleseremn鋤tra缸wereinducedtoacuteIiver師lu他byDGalac

6、tosamineandwercdiVidedr甜domIyjntoGroupcandGroupDThenthesetWogroupsweretranspl孤【edbythes姍emcthodsasthoseinGroupAa11dGfoupBrespectiⅥlyTheliv盯缸1ctionind啦theAFPvalwsandllverhst0IogyofraTsinGroupCandDhavebeenex鋤jncdbyradiOimm

7、unoassayandhlstologicaltechn0109y_Results：1、survIValratcofgm“pAwashl曲erthanmatofgroupB(63，16％vsl500％，P‘005)2、111erewassignincantdj髓rencejnAFPvaluebe眥enGrouPCandGroupD，andsignmcantdi位rencealsoliesinthegroupsbefore柚da髓rtra

8、ll5pl皿呲ion(P005)，肌dbetweenth。gm“psbefore鋤daRer仃an9plantation(P005)4、LivorhiscuIogy。f0roupcw船mo∞improvedthanthalofGro“pDTherewaS劉gni6cantdj竹erenceinhepatjcovalcellnuInbersbe”eenGroupC甜1dGroupD，andsignincanIdi腩rencealso1ie

9、sinth。g’o“psbetorea【ldaRernnsplarItatIon(PO05)ConcIusioⅡ：1、SuⅣivalratealldtheliverhi5tologyofratssufrerJngfmmacmeliverfailureinducedby口Galact05鋤inewereimprovedviain昀pe血onealh。patocytetrallsplanta_clOn2、Hepatic0vmcollsOfn

10、onadjvatjonandactivationa缸r1ver筋lurecouHbenimulatedbythesetransplalltedhoPatoc”es孤dthe盯prolifcratlonanddlffe陀ntiationcou“bespccded工heycouldexe^aIlimporcante丘＆tont1ereversionofliverfjilureKeywords：hepaIo。ycehepatic。valcel