鼻咽癌血清的拉曼光譜研究.pdf

1、福建醫(yī)科大學碩士學位論文鼻咽癌血清的拉曼光譜研究姓名：陳奇松申請學位級別：碩士專業(yè)：腫瘤學（腫瘤放射治療）指導教師：潘建基林少俊20100401ResearchonRamanSpectraofSeruminNasopharyngealCarcinomaAbstractobjective：TesearchontheapplicationvalueoflaserRamanSpectraforserumscreeninganddiagnosi

2、sinearlynasopharyngealcarcinomaMethod：17casesofhealthyvolunteersand29casesofnewlydiagnosednasopharyngealcarcinomabypathologywereselectedinOHFhospitalfromMay2009toDecember2009Peripheralvenousbloodsamplesweretakenfromallsu

3、bjectstotubesAllbloodsampleswerecentrifugedtemovebloodcells；seroussampleswereobtainedinthesupernatantInViaconfocalRamanmicroscopewasappliedtotestthesamplesForeachsample，wetested5spotsfor5RamanspectraTheaverageof5spectrai

4、nonesamplerepresentsthesignalofRamanSpectrainonecaseThen，RamanspectraofNPCpatientsgroupandhealthyvolunteersgroupwereanalyzcdbystatisticalmethods，includingttest，principalcomponentanalysis，discriminantanalysisMoreoverabove

5、statisticalmethodswasappliedtoanalyzeRamanspectraoftwosub—groupofEBVDNAnegativeandEBVDNApositiveinNPCpatientsResults：Theaverageofspectrumpeaksincomparedtwogroupsisalmostthesame，whilethereissignificantdifferenceoffrequenc

6、yshiftinmajorspectrumpeaksbetweentwogroupsIntensityofcommonspectrumpeaks11445，116510andratioof11445／11651arenotstatisticallysignificant(relatedPvaluesare“008，051and041)Moreover，accordingtothediscriminantanalysisfollowedb

7、yprincipalcomponentanalysis，specificityorsensitivityofretrospectiveanalysisbetweentwogroupsis100％，probabilityoferroriszeroComparedtocrossanalysis，sensitivityis100％，specificityis931％(27／29)，probabilityoferroris43％(2／46)Me

8、anwhile，forthetwosub—groupsofEBVDNAnegativeandEBVDNApositive，sensitivityofretrospectiveanalysisbetweentwogroupsis917％，specificityis909％，probabilityoferroris87％(2／23)Comparedtocrossanalysis，sensitivityis417％(5／12)，specifi