PTEN基因在腦膠質(zhì)瘤表達(dá)的研究.pdf

1、青島大學(xué)碩士學(xué)位論文PTEN基因在腦膠質(zhì)瘤表達(dá)的研究姓名：尹衛(wèi)寧申請學(xué)位級別：碩士專業(yè)：神經(jīng)外科學(xué)指導(dǎo)教師：孟慶海2003.3.10StudyonPTENgeneexpressioningliomaAbstractObjectivePTENasatumorsuppressorgeneplayedanimportantroleinthegenesisanddevelopmentofgliomaSomestudiesonPTENmostly

2、focusedongenemutationButmutationcannotexplainthemechanismofgliomacompletelYThiSstudyaimstomakeclearthemechanismofgliomaandtheclinicalsignificancebytheinvestigationintheexpressionofgeneingliomaInaddition，thiSstudyalSOtrie

3、stoevaluatetherelationshipbetweentheextonunexpressionandgliomagradeMethodsTheexpressionofPTENmRNAwasdetectedbyreversetranscriptionpolymerasechainreactionin45casesofgliomasand15casesofuormalbraintissuesResultsTherespectiv

4、elynegativeexpressionrateofPTENmRNAwas556％(extonl一8)，5335(extonl一9)，556％(exton5—8)，578％(exton5—9)TherewasnonegatireexpressioninnormalbraintissuesTheexpressionofPTENmRNAdidnotshowcorrelationshipwithsuchfactorsasage，gende，

5、butwiththehistologicalgrade(pO05)ConclusionTheDNAmutationwasnottheonlykeyfactorinthegenesisofgliomtaSomeabnormalproductsofPTENgeneperhapswasnotrelatedwithDNAmutation，butitwasrelatedwiththeabnormaltranscriDtionwhichledtot

6、heinactivationsofproteinThenegativeexpressionsofPTENmRNAweremostlYfoundinthemiddleandlatecourseofgliomas，whichindicatedthattheabnormaltranscriptionplayedanimportantroleinthegenesisanddevelopmentofgliomasKeywordsPTENgeneg