人膽管癌組織原發(fā)灶和轉(zhuǎn)移灶裸鼠種植模型的建立及觀察.pdf

1、分類號UDC：Y941937密級：請注明密級及保密期限石河子大學(xué)碩士學(xué)位論文人膽管癌組織原發(fā)灶和轉(zhuǎn)移灶裸鼠種植模型的建立及觀察研究生指導(dǎo)教師申請學(xué)位門類級別學(xué)科、專業(yè)名稱研究方向所在學(xué)院劉斌馮德元教授中國新疆石河子2006年6月Establishmentofanimalmodelinnudemicebyretransplantationhumaneholangiocarcinomatissueintosubcutaneousandobs

2、ervationPostgraduatestudent：LiuBinSupervisor：ProFengDeyuanAbstractOBJECTION：ToestablishaxcnografiedmodelofhilarcholgangiocarcinominnudemiceMETHOD：A丹eshhumanhilarcholangioeareinomaspecimenwasobtainedfromapatientunderasept

3、icconditionduringoperationincludedprimaryandmetastatictissueUsingpartsofprimarytissueodivideditintoinner，middleouterthreeparts，quicklycuttingtheseintolmm’smallpieces，withmnastafictiSSUeandrandomlytoestablishprimary(mer)，

4、primary(middle)，primary(outer)andmetastaticfourgroupsanimalmodels，andwillietransplantatiandobservefromgenerationtonextgeneration(total3generations)；ThetnnlorspecimenWascutintopieces，culturedin1640medium，andsubcutaneously

5、lxansplantedintoBulb／cnu／unmiceWhenthetmnorsreached10nllnindiameter，orthehostshowedcachexia，thetransplantedtumorswereretransplantedintonewmiceThemice、vereobservedevery5daysandthegrowthOfl：lImorwasrecordedandgrowthcmveswe

6、refiguredThemiceweresacrificedwhentheyshowedseriousillness，andlaparotomizedtoexminemetustatictunlorsinliverandlungsDhectdissemin面onmethodWasusedtoaffirmthekaryotype；vascularendothelialgrowthfactorfvEGF)，basicfibroblastgr

7、owthfactor(FGF2)，proliferatingcellnuclearantigen口CNA)WasexaminedⅥTitllimmunohistochemicalmethodsFlowcytometrywasusedtoexamineeveryperiodSpercentageofcellcycle：Carbohydrateantigen242(CA242)，carcincembryonicantigen(CEA)Wer

8、eexaminedinradioimmunoassay，RESULT：Thettanorwasmobile，aliflestifandsecretedamountsofmucusHEstainingshowedapoorlydifferentiatedadenocarcinomas仇lc帆，justlikethehumanhilarcholangiocareinomaspecimenThetumorWassuccessfullypass

9、aged3timesTheincnbationperiodWasshortenedtb34daysandthetmnorgrewfasterthanthefirstpassageNoliverorlungmetastatictunlorswereobservedinallthemiceVEGFFGF2，PCNAwereheavilyexpressedFloweytometryexaminationshowsthattheratioofS

10、phasefractioninmetastatictissueishigherthanthatinprimarytissueAccordingtoradioimmunoassay，thecellularconcentrationsofsometumormarkerswereasfollow：thetwomarkerswereallnegativeinSenlmExceptthefirstpassagegroachcnrve，otherp