野敗型CMS育性恢復基因的微衛(wèi)星標記定位.pdf

1、中國農(nóng)業(yè)科學院碩士學位論文野敗型CMS育性恢復基因的微衛(wèi)星標記定位姓名：李廣賢申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：莊杰云姚方印20050601AbstractCytoplasmicmalesterility(CMS)anditsrestorationarcbasesforthebreedingandcommercialapplicationofthreelinehybridriceWildabortiveCMS(CMS

2、WA)WaSfirstlyusedinthecommercialproductionofhybridriceandhasalwaysbeenmostextensivelyappliedInthepresentstudy704linesofthetestcrosspopulationZhenshan97A／(Zhenshan97B／Milyang46)F6wereestablishedformappingrestorergenesforC

3、MS—WA，SSRmarkerbasedgeneticlinkagemapWaSconstructedtocoverchromosome10andtheshortarmsofchromosomesland11inwhichgenesforCMSfertilityrestorationhavebeenreported肼髓踟originalpopulationconsistingof704lines，fourQTLsforCMS一腑fert

4、ilityrestorationweredetectedamongwhichRf4onthelongarmofchromosome10displayedamajoreffectRf3ontheshortarmofchromosome1hadaconsiderableeffect，andg明0andqef／linthepeficen仃omericregionsofchromosomes10and1l，respectivelyhadmino

5、reffectsSignificantdigenicinteractionWasalsodetectedbetweenRf4andRf3，showingpartiallyduplicateactionTodeterminetheeffectofeachrestorergeneintheabsenceofRf4orR療，thepopulatiOnWasclassifiedintotwosubpopulatiortsbasedongenot

6、ypesatRM6100andRMll95whichwerecloselylinkedto明andRf3，respectivelyInonesubpopulations，thegenotypeatthegivenmarkerlocusWasmaternalhomozygotesandthecorrespondingfertilityrestoringalleleWasabsent，andtheothersubpopulationshad

7、heterozygotesatthegivenmarkerlocusindicatingthepresenceofthecorrespondingfertilityrestoringalleleEachofthefoursubpopulationsWasusedtodetectQTLsforfertilityrestorationRf4showedmajoreffectsforfertilityrestorationintheabsen

8、ceandpresenceofRf3TheotherflueeQTLshadeffectsforfertilityrestorationintheabsenceofRf4amongwhich膨hadthelargesteffectItWasalsofoundthat8p，qefloandqRfllcontributedtohigherspikeletfertilityinthepresenceofRf4，whereasqRf，oandq