CEA蛋白、CEA-mRNA在大腸癌、胃癌組織表達的研究.pdf

1、青島大學碩士學位論文CEA蛋白、CEAmRNA在大腸癌、胃癌組織表達的研究姓名：劉中路申請學位級別：碩士專業(yè)：免疫學指導教師：高美華20090602InvestigationofCEA，CEA—mRNAExpressioninColorectalCancerandGastricCarcinomaTissuesABSTRACTObjective：ToexploretheepressionofCEA，CEA—mRNAincolorectal

2、cancer’gastriccarcinomatissues，InordertoprovidethetheoryofearlydiagnosisofcolorectalcancerandgastriccarcinomaMethods：TheserumCEAlevelswereestimatedin21patientswithcolorectalcancer20patientswithgastriccarcinomaand20patien

3、tswithoptimumgastrointestinaldiseasebeforebeingoperatedAndexpressionratesofCEA，CEAmRNAincarcinomatissues，adjacenttissuesofcarcinomaandoptimumgastrointestinaldiseasetissueswereestimatedbyimmunohistochemicalmethodandrealti

4、mefluorescentquantitativeRTPCRmethodResults：TheExpressionratesofCEAinserum，carcinomatissuesandadjacenttissuesofcarcinomaincolorectalcancerwere619％(12／21)，952％(20／21)，143％(3／21)；andthatingastriccarcinomawere50％(10／20)，80％

5、(16／20)，and10％(2／20)TheExpressionratesofCEAmRNAincarcinomatissuesandtheiradjacenttissueswere100％(21／21)，33％(7／21)incolorectalcancerandwere100％(20／20)，30％(6／20)ingastriccarcinomaOutofthepatientswithoptimumgastrointestinal

6、diseasetheserumCEAlevelwas35％(7／20)，andtheExpressionratesofCEA，CEAmRNAintissueswere15％(3／20)，30(6／20)ThepositiveratesofCEA—mRNAexpressioningastriccarcinomatissuesdiffersignicantlyfromthatofCEAinserumandincarcinomatissues