Ag-Ab-PEI-DNA病毒狀顆粒的研究.pdf

1、Gene delivery-based on non-viral DNA vehicle is an attractive approach than viral vector. Several synthetic non-viral DNA transfer systems have been developed to overcome physiological, as well as intracellular barriers.

2、 However, in vivo gene transfer efficacies with non-viral vectors have been rather low in comparison to viral vectors. The ideal non-viral vector for gene delivery should condense DNA into neutral complexes,avoid toxicit

3、y interactions with cells, mediate cell specific binding and avoid DNA degradation in acidic vesicles. Targeted delivery of non-virus vectors into cells is likely to improve the delivery efficiency. Galactosylated polyet

4、hyleneimine has been used to increase the transfection efficiency of DNA in hepatoma cells. And antibody-mediated targeting of particles and lipid-mediated targeting of DNA have been exploited to increase the transfectio

5、n and gene delivery efficiency.Taken together, the complexes of Ag/Ab-PEI and DNA have the ability of self-assembly into Ag/Ab-PEI/DNA VLPs. This kind of Ag/Ab-PEI/DNA VLPs can be used to effectively transfect Huh7 cells