胺苯磺隆解毒劑的資源篩選與機(jī)理研究.pdf

1、湖南農(nóng)業(yè)大學(xué)碩士學(xué)位論文胺苯磺隆解毒劑的資源篩選與機(jī)理研究姓名：劉祥英申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)藥學(xué)指導(dǎo)教師：柏連陽(yáng)20050501ABSTRACTAimingattheinjuryproblemofethametsulfurontosucceedingricewescreenedacertainantidotefromherbicidesfungicidesinsecticidesandplantgrowthregulatorswhi

2、chhavebeenwidelyused.ThispaperdescribedtheantidotalefectofNtoethametsulfuronusingbioassaymeasureintheroombioassaymethodsingreenhousesimulatedethametsulfuronresidualexamandthemechanismofNforreducingthephytotoxicityofetham

3、etsulfuononricewasdoneonthebaseofformerstudies.Themainresultswerelistedasfollowed:1.Tostudythesensitivityoffivediferentricespeciestoreacttoethametsulfuonitcanbefoundliangyoupeijiuwasthemostsensitiveamongthefivericespecie

4、sandtheIC50ofricemainrootlengthofliangyoupeijiutoethametsulfuronwere2.48mgLinwaterand3.03pgkginsoilrespectively.2.Acertainantidotehadbeenfirstlyselected.Theantidotalefecttestshowed:Ncanalleviateharmfulefecttothesucceedin

5、griceattheconcentrationbetween0.5ggkgand40ggkgwhensoilethametsulfuronwasIggkgwithlOpgkgbeingtheoptimum.ThelOggkgNcanreducethephytotoxitytodiferentextentwhenethametsulfuronwasunder20pgkginsoil3.Greenhouseexperimentdemonst

6、ratedthatNcanprotectthesucceedingricefrom0.1201tgkgethametsulfurondamage.Thericeyieldincrease6.3%12.3%21.2%23.7%40.4%respectivelywhendiferentethametsulfurontreatmentmixedlOggkgNinsoil.AtthesametimeNhadlitletendencytoacce

7、leratethedegradationofethametsulfuron.4.ThemechanismofNreducingthephytotoxicityofethametsulfurontoricewasstudiedthroughthephysiologicalandbiochemicalexperiments.TheresultsshowedthattheNincreasedchlorophyllcontentrootacti

8、vityGSHcontentandGSTactivity.ButithadlitleinfluenceonALSspecificactivityinvitroandinvivo.ThereforeonepossiblemechanismwasthatNincreasedricephysiologicalefectinadversity.Theotherplausiblemechansimmaycatalysetheconjugation