小麥組織培養(yǎng)再生系統(tǒng)的優(yōu)化及DREB1A基因的轉(zhuǎn)化.pdf

1、中國(guó)農(nóng)業(yè)科學(xué)院碩士學(xué)位論文小麥組織培養(yǎng)再生系統(tǒng)的優(yōu)化及DREB1A基因的轉(zhuǎn)化姓名：趙林姝申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：劉錄祥20050601AbstraetWheattransformationviamicroprojectilebombardmentwithimmatureembryoculturewasstronglydependedonthegenotypesThisproblemrestrictedthewide

2、applicationoftransgenicwheattoagreat％tentOptimizationofregenerationsystemofwheattoovercomethegenotypeobstaclehasgreatsignificanceinpromotingtheapplicationoftransgenicwheattoalargescalenli8studyemployed3winterwheatgenotyp

3、esH6756H311andSP8581tocomparetheeffectofsamplingtime，callusinductionmedia，differentiationmediaandroofingmediaonwheatyoungspikecultureTheresultsshowedthathi【ghfrequenciesofgreenplantletdifferentiationwereobtainedwhenyoung

4、spikeswereculturedbetweentheformationstageofprotectiveglumeprimordiumandformationstageofpistilandstamenpfimordiumTheoptimummediaforcallusinductionWaSMS2，4D2mg／L，forgreenplantletdifferentiationMS，andforrooting1／2MS02mg／LI

5、AA809／LsucroseAnefficientregenerationsystemforyoungspikecultureofwheatWaSsuccessfullysetupByuseoftheestablishedwheatregenerationsystem，youngspikesandimmatureembryosof17genotypesofwheatwereinvitroenlturedtocomparethecallu

6、sinductionfrequenciesandgreenplantletdif婦en矗ationfrequenciesThe2yearsresultsshowedthattnerew∞nosigaificamdifferenceincallusinductionfrequencybetweenyoungspikesandimmatureembryosofalltheinvolvedgenotypesButthegreenplantle

7、tdifferentiationfrequencyofyoungspikesWashigherthanthatofimmatureembryosill15genotypesoutofallthe17genotypes，indicatingthatyoungspikewasagoodexplantinwheatinvitrocultureDREBlAgeneWastransferredviamicroprojectilebombardme

8、ntintocallifrombothproperstagedyoungspikesandimmatureembryosofH6756andH311，whosefrequenciesofgreenplantletregenerationfromimmatureembryocalliwerelowerthan20％Asaresult，thefrequenciesofgenetransformationofH311andH6756tothe

9、iryoungspikeaccepterwere256％and588％respectivelyWhilenotransgenicplantswereobtainedinbethH311andH6756whentheirimmatureembryocalliwereusedasgeneacceptorsThedroughttoleranceofT3transgenielinesofH6756withD1LEBlAgeneWaSidenti

10、fiedduringthegerminationandyoungplantgrowingperiodsAlthoughsometransgeniclinesshowedinconsistenceofdroughttoleranceatgerminationandyoungplantgrowingperiods，threetransgeniclines，H67561520H6756818andH6756—515wereselectedfo

11、ralltheirdroughtresistantindexeshi曲erthanthecontrolatbothgerminationandyoungplantgrowingperiodsSuchresultssuggestedthatthesethreeWansgenicwheatlines0fH6756withDREBlAgenehadimproveddroughttoleranceKeyWords：Youngspikeofwhe