VD-toxin作用下擬南芥懸浮細(xì)胞微絲骨架和核仁的變化.pdf

1、Y774841分類(lèi)號(hào)：密級(jí)：。皎罌?！雴挝淮a：t0019學(xué)號(hào)：S02364飛嚼差≮夭等’碩士學(xué)位論文VDtoxin作用下擬南芥懸浮細(xì)胞微絲骨架和核仁的變化Vertidl『覷mdah／／aeToxinInducedAlteratior醛ofActinCytoskx4etonandNucleolushlArab／dops／sSuspensionCells研究生：袁篷丞指導(dǎo)教師：奎題童申請(qǐng)學(xué)位門(mén)類(lèi)級(jí)別：理堂亟專(zhuān)業(yè)名稱(chēng)：撞塹堂研究方向：緝跑值

2、墨鰱昱所在學(xué)院：生物堂瞳2005年6月AbstractInplantcells，actinc”oskeletonplaysimportantrolesinresponsetobioticand曲ioficstressesHowever，littlehasbeenknownaboutthedynamicsofactincytoskeletonwhencellsareattackedbyunphysicalattacksfromsomest

3、ressfactorssuchaselicilotsWereporthemthatthetoxinofVerticilliumdahliae(vDtoxin)inducedthechangesofactinfilamentsinArabidopsissuspensionculturedcellsWhenthecellswemtreatedwithlowconcentrationVDtoxin，F(xiàn)actinarraysweredismpt

4、cdordinallyfromCOrtextonucleusaround，andthencametheautomaticrecoveryofthemTheactin蜘amentsintlleperinuclearregionshowedmoreresistancetoVDtoxinthanthecorticalonesIncontrast，whenthecellsweretreatedwithhJighconcentrationVDto

5、xin，F(xiàn)actinarraysweredismpledmoreseverelyandthedisruptedaOincytoskeletoncouldnotberecoveredspontaneouslyThetreatment0fhjghconcentrationVDtoxinoncellsalsoinducedthechangesofnucleofiAttheearlystagesofthetreatment，anucleusha

6、dasingleringshapednucleolusAtthelaterstages，multiplesmallerandbrjglllernucleoliemceedinasinglenucleusDismptedactincytoskeletoncouldberecoveredbyremovaloftheVDtoxinbeforethenucleoliweredyedAlltheseresultsshowedthattheacti

7、ncytoskeletonplaysimportantrolesintheearlydefenseresponsesagainstVDtoxininArabidopsissuspensioncellsThechangesofthenucleoliinducedbyVD—toxinmaybeimportantcharacteristicsthatwereaccompaniedbyresistancerelatedcelJdeathKeyw