DNA指紋技術(shù)在中國三種實驗用小型豬分子遺傳檢測中的應(yīng)用.pdf

1、Objective: 1. To explore if ECL (Enhanced ChemiLuminescene)direct nucleic acid labelling and detectionsystems can be used in the fingerprinting of pig genomic DNA;2. To detect the inbreeding degree ofbanna miniature pi

2、gs with DNA fingerprinting and STR.3. To study the genetic background of the three strains of minipigs with DNA fingerprinting andSTR.Conclusions: 1. ECL can be used to DNA fingerprinting of pigs and the same effect can

3、be achieved to thosegenerated from the isotopically labelled probe.2. The two probes are available to the DNA fingerprinting for Banna miniature pig and obtain thesame result.3. The similarity coefficient in families is

4、higher than that between families.4. similarity coefficients of family "805" in substrain JB and family "151" in substrain JS werethe highest.this showed that the two families have the higher inbreeding degree, smaller g

5、eneticdifference among individuals and the better homogeneity.5. various endonuclease will produce various DNA fingerprint fragments,but generate the samesimilarity coefficient. This also showed that the two kinds of min