24194.觀賞鳳梨優(yōu)質(zhì)品種收集以及遺傳轉(zhuǎn)化研究

1、浙江大學(xué)農(nóng)業(yè)與生物技術(shù)學(xué)院碩士學(xué)位論文觀賞鳳梨優(yōu)質(zhì)品種收集以及遺傳轉(zhuǎn)化研究姓名：沈曉嵐申請學(xué)位級別：碩士專業(yè)：風(fēng)景園林指導(dǎo)教師：毛碧增王煒勇20100610浙江大學(xué)碩士學(xué)位論文觀賞風(fēng)梨優(yōu)質(zhì)品種收集以及遺傳轉(zhuǎn)化研究Abstract：Bromeliads，theexcellentindoorornamentalfoliageandfloweringplants，itsconsumptionrosesharplyinrecentyears，w

2、hiletherearelimitedresourcesinChinaTheintroductionofforeigngenesbygenetictransformationprovidesallalternativeapproachtoenrichgermplasmresourcesInthisstudyweevaluatedagermplasmcollectionandestablishedtheparticlebombardmen

3、tedmediatedgansformationsystemforBromeliadsMorethan661germplasmswereobtainedandevaluated，amongtheseGuzmania‘Diana’andG‘Focux’weredonatedastransgenicmaterialsThehighefficiencyDNAextractionmethodwasestablishedthroughextrac

4、tionliquid，material，grindingmethods，settlingtime，andSOonindifferentbromeliadsspecmsWealsoestablishedtheefficiencymicropropagationsystemthroughttipandsheathofGuzmania’TutteFrutte’Theresuhsindicatedthatthebestinductionmedi

5、umwasMS6BA05mg／LNAA05mg／LthebestdifferiationmediumwasMS6BA20mg／LNAA05mg／L2，4一D20mg／LandthebestrootinductionmediumwasMSliquidmediumThestudyofmicroprojeetilebombardmentmediatedgenetictransformationforGuzmaniawascarriedoutw

6、iththeshortenstemof’Diana’and’Focux。andthreepositivelineswereobtainedandconfirmedbvPCRTheexpressionofpBIN438一GFPinthetwokindsofGuzmanialeavesWasobservedundertheLaserscanningconfocalmicroscopeTheresultsimpliedthatthemicro

7、projectilebombardmentmediatedgenetictransformationsystemfortheornamentalbromeliadsWasestablishedandprovidesanalternativeapproachtoenrichornamentalbromeliadsgermplasmresourcesKeywords：ornamentalBromeliads，Guzmania，genetic