禾谷類作物谷胱甘肽轉(zhuǎn)移酶與除草劑抗性相關(guān)性的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文禾谷類作物谷胱甘肽轉(zhuǎn)移酶與除草劑抗性相關(guān)性的研究姓名：徐剛申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)藥學(xué)指導(dǎo)教師：陶波2003.6.1，，，一。。，一。，。一。。，，。至苧耋悲查蝥薹霪邕簍ir_。。I。一。，。。。。一。。StudiesonRelationshipbetweenGlutathioneS—TransferaseandTolerancetoHerbicidesinGraminealCropsAbstractThere

2、atemanyconcelTlStosomeseriousproblemswiththeuseofherbicidesRecentlywithadvancedacknowledgeintheGSTcatalyzedGSHconjugationfordetoxi句ingsome趨efbicides。moreeffortsafeneededto蘸OsomeresearchesOnit。nlesmdyusedbiologicalandmath

3、waytoanalyzethecrops’tolerancetoacetochlororatrazinethi∞ughdecidingthedifferentherbicideconcentrationthatCallinhibit50％plantshootheightGSTactivitiesincropswasanalyzedinHabigwayandGSHlevelswaSanalyzedinJeanwayGSTpolyzcryl

4、amidegelelectrophoresisWaSperformedtoshowcompoun瓠ofisoenzymesindifierentcropswithandwithOUthe南i娃detreatment。1Theresultsshowed：withthetreatmentofacetoehlorthecurveshadasinglepeakthatusuallyreachedat3thdayGSTactivitiesandG

5、SHlevelsincropshadsanlesequencewiththeirtoleranceagainstacetochlor曩”sequenceofmaizecultivars’wasDongnon9248Dongnon99702DongnongWannianDongnongBaoLieDon群ianN0311lesequenceinsorhumWaSTongzaN02Ao∞N02R741；InglasswaSEtoubaiYu

6、guInsalnecrop1∞sequenceofGSTactivitieswashJighconcentrationsmiddleconcentrationslowconcentrations2，Withthetreatmentofatrazine。氌oouⅣ％hadsinglepeakthatusuallyreachedat2thdayGSTavtivitiesincropshadsamesequencewiththeirresis

7、tancetoatrazineThesequenceinmaizew粥Dongnon9248DongnongBaoLieDongnon99702DongnongWannianDongtianN03。InsorghumcultivarsthesequenceWaSTongzaN02G741AozaN02。ingrassthesequencewasEtoubaiYuguInsamecropthesequenceofGSTactivities

8、washighconcentrationsmiddleconcentrationslowconcentrations。3TheGSTactivitiesandGSHlevelshadsamecorrelativeratewiththecroptolePanceagainstacetochlrinmoderotelevelGSTactivitieswerehighlyrelatedwithtotheirtoleranceagainstat

9、razine。ButGSHlevelshaddefinitelytowcorrelativeratewiththeirtoleranceagainstherbicides4。GSTisocnzymesineverycropcultivarsshowedconstitutiveGSTwithouttreatmentwemdifirerentincropcultivarsWiththetreatmentofacetochlorandalra