三種蕨類植物的離體保存技術(shù)研究.pdf

1、辛中巖毒夫?qū)W研究生學(xué)位論文論文題目三種蕨類植物的離體保存技術(shù)研究研究生姓稻：盤文濤導(dǎo)師姓名：陳龍消教授專業(yè)：疑婪鑿墼。申請學(xué)位。級別壩|_=研究方向：瓣嫠簍黎蓑壅督翥：2003f2003fI8一YJ提變悶期：I中國武漢ABSTRACTTheobiectireofthisresearchistOestablishatechniqueastofindoutabestwayforDresenringfernsefficientlyThemat

2、erialsarespore、pratholliumsporophyteofAdianmmrenifovineLvatsinese；Adiantumcapillus—venerisandPlatyceriumwallichiiInordertOdiscovertheeffectofdifferentpretreamaentatdifferenttemperatureonfern‘Sphysiologicalandgeneticchara

3、cterizationtheyhadbeenpreservedatthetemperatureof4℃一72Cand，196℃anddifferenttimeforlday7daysand30daysrespectivelyThecontentofthisresearchincludes：thesurivalrateandgerminationofsporeafterpreservedbylowtemperature；theactivi

4、tyanddefferentiationofprothalliumafterpreservedbylowtemperature；thesurvivalrateofsporophyteafterpreservedbylowtemperatureandthemicropropagationsystemforAdiantumcapillus—venerisandPlatyceriumwallichiiafterthe)arepreserved

5、bylowtemperatureTheresultsasfollow：1Theviabilityofthreespeciesoffem‘Ssporeiskeptatthetemperatureof4“Cand一72。Cdecreasedwhenthepreservationtimeprolonged；at一196“C，itchangedslightlyTwomethodofthawingdidn‘tproduceobviouslyeff

6、ectonthesurvivalrateofthefernsspore2WhenprothalliumofAdiantumcapitlus—venerisandPlatyceriumwallichiihadbeenkeptattemperatureof44Cand72℃fordifferenttimes，theirsurvivalratedecreasedrapidlywhenfreezingtimeprolongedAndunderc

7、ryopreservedbyvitrificationthesurvivalratioCallgettOahighlevelanddidn’tdecreasedwithtimeelapsedThequicklythawingmethodhasbenifitedtOthesurvivaloftheprothallium3Preservedinliquidnitrogen(LN)thesurvivalratioofsporophyteCan

8、reachmorethan80％andrapidthawinghasadramaticeffectonitSameasprothallium，preservatedin4。Cand72“Cthesurvivalrateofsporophytedecreasedrapidlywhenfreezingtimeprolonged4ThetechniquessystemsuitabletOcryopreservationforPteridoph

9、yteareasfollows：ThelongtimepreservationforsporeshouldkeepinLN，andshorttimepreservationshouldkeepat44Candthetimewouldbetterlessthan1monthAndtOspore，thetW0thawingmethodcarlbeusedThewayofpreservationofprothalliumandsporophy

10、teissarnetosporebutthewayofrapidthawingisbetterforgettingahigherviabilityItneedatreatmentprocessforthematerialscryopreservedbeforetheyareplungedintocryopreservationequipmentFirsttheyshouldbeloadedwithcryoprotectantfor20t