中草藥抗仔豬大腸桿菌性腹瀉作用機理的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文中草藥抗仔豬大腸桿菌性腹瀉作用機理的研究姓名：劉玉芹申請學(xué)位級別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：張秀英2002.5.1StudyonMechanismofActionofChineseHerbalMedicineonEscherichiacoilinducedDiarrheaofPigletABsTRAcTInthispaperthemechanismofactionofthefiveChineseherbalm

2、edicineswhichareIsatisleaflsatisrootForsythiafruitPatriniaandBerberinehydroohloride，andtheircomplexprescriptionsonEscherichiacoil—induceddiarrheaofpiglet，weresystematicallystudiedfromfiveaspectswhichafethebacteriostatica

3、ctivityinvitroonEcoil。lheantagonismagainstliquidretentionmediatedbyEscherichiacoliheat1ablieenterotoxin(LT)，theantagonisticeffectondiarrheainducedbycastoroilinmicetheinhibitingactiononincreaseofcapillarypermeabilityinduc

4、edbyaceticacidinmouse’sabdominalcavitytheinhibitingeffectonintestinalmovementforwardinmice：Thebestcomplexprescriptionwithallgoodactivityagainstbacteriaantagonismagainstenterotoxinantiinflammatoryactionandantidiarrheaacti

5、onwasscreenedoutsothesecanprovidetheoreticbasesfortheselectionofeffectivemedicinetotreatEscherichiacoilinduceddiarrheaofpigletandthedevelopmentofthecomplexprescriptioncomposedofChineseherbaImedicineandantibacteriaIagents

6、Theresultsofthebacteriostaticexperimentjnvitroindicatedthatoutoffivesinglemedicines。exceptBerberinehydrochloride，lsatisleaflsatisroot，F(xiàn)orsythiafruitandPatriniahadobviousbacteriostasisThebacteriostasisofIsatisrootinvitrow

7、asthebestandreachedO00169，ml(MIC)and0003lg／ml(MBC)respectivelyAmongthecomplexprescriptions，thebacteriostasisofComplexprescriptioncomposedoflsatisrootForsythiafruitandPatriniawasasgood∞thatcomposedoflsatisleaflsatisrootan

8、dForsythiafruit。andthevitroactivityofthesetwocomplexprescriptionswerestrangerthanComplexprescriptioncomposedofForsythiafruitandPatriniaThevitroactivityofComplexprescriptioncomposedofIsatisrootForsythiafruitPatriniaandBer

9、berinehydrochloridewaspoorEscherichiacoliwasculturedinbacteriaIculturemediumtowhichtheextractsoftheChineseherbaJmedicineswereaddedandtheculturalproductLTwasusedtodoexperimentinligatedrabbitileum1satisleafIsatisrootandFor

10、sythiafruitsignificantlyorhighsignificantlyreducedtheliquidretentioncausedbyculturalsupematantfluidinligatedloops(P《005，P005，P001)AntagonisticeffectsofComplexprescriptioncomposedofIsarisrootForsythiafruitandPatriniaandCo