人工感染綿羊肺腺瘤病毒內(nèi)蒙株羔羊的臨床病理學(xué)研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文人工感染綿羊肺腺瘤病毒內(nèi)蒙株羔羊的臨床病理學(xué)研究姓名：武志紅申請學(xué)位級別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：馬學(xué)恩20090501StudiesontheClinicalPathologyofSheepExperimentallyInfectedwithJSRvNMStrainAbstractOBJECTIVE：Toexaminetheeffectsofjaagsiektesheepretrovirus(JSRVN

2、M)ontheclinicalpathologicalmanifestationthechangesoflymphocytessubsets，lyrnphocytesapoptosis，bloodgasofsheepexperimentallyinfectedwithJSRVNMstrainMETHODS：InMarch2008fivenewbornlarabswithin24hoursaftertheirbirthwereinocul

3、atedintratracheally、忻也cellfreefiltrationofpulmonarytUl：nOUrtissuecontainingJSRVNMstrainfromnatu捌cases10mlperlambAtdifferentpostinfectionveinbloodsamplesWel“ecollected，andthelymphocytesapoptosisnumberCD4TandCD8Tlymphocyte

4、swereexaminedbyusingtheflowcytomytorAtthesametime，thepH，pC02，p02BE(Baseexcess，)，BB(Bufferbase)，HC03std，HC03actTC02(T0talcarbondioxide)，cH(hydrogenionconcentration)，02SAT(02saturation)，02Com(Oxygencontent)，ctHb(est)、AnGap

5、，K(Kaliumionconcentration)，Na(Sodiumionconcentration)，C1(Chlorineionconcentration)，andtheactivityofCKLDH，ALP觚ASTwereinspectedRESULTS：nleexperimentalgrouppresentedsomeclinicalsignsbfthesheeppulmonaryadenomatosis(SPA)Thebo

6、dytemperatureWasnormal，thepulserateandthebreatheratehigherthanthenormalrangeAt30dpost—infection，therelativepercentageofCD4TlymphocytesandtherelativepercentageofCD8Tlymphocyteswerehigherthenthecontrolgroup，anddecreasesubs

7、equently，andthenshowafluctuationAt30dpost—infectiontheearlyandlatelymphocytesapoptosisnumberlowerthanthecontrolgroup，undulatingintheaftertime。Indifferenttimeperiods，AnGap，p02，HC03act，HC03std，ctC02，02SAT，02CT，Hct，ctHbtest

8、)ofanalysisofbloodgasandALT，AST，LDH，CK，ALPofserumenzymesshowedsignificantdifferencebetweentheexperimentalgroupandthecontrolgroupCONCLUSIONS：netypicalsymptomsofSPAhasnotappeared，andthediseaseWasintheinitialstagemchangesof

9、CD4TCDgTlymphocytesinperipheralbloodofsheepexperimentallyinfectedwithJSRVNMstraindemonstratedwhichmaybetheresultofcellimmunologicalreactionandthelymphOCytes測uryPeripherylymphocyteapoptosisinsheepexperimentallyinfectedwit

10、hJSRVNMstrainWasfound，lymphocyteapoptosismaybeolleofthemostimportantclinicalsymptomsnledetectionofblood—gasandtheserumenzymesofsheepexperimentallyinfectedwithJSRVNMstrainwashelpfulfordiagnosisofSPAKeywords：Experimentally