擬南芥總蛋白提取

1、河北經(jīng)貿(mào)大學(xué)畢業(yè)論文擬南芥蛋白不同提取方法的SDS—PAGE凝膠電泳分析專業(yè)名稱：生物技術(shù)班級：200302學(xué)生姓名：陳麗麗指導(dǎo)教師：陳穎完成時間：2007年6月河北經(jīng)貿(mào)大學(xué)畢業(yè)論文IAbstractTherearemanymethodsofthewithdrawtionoftheplantproteinsnomatteranymethoditmustpreservethebiologymacromolecularstructureto

2、beintegrityintheoperationpreserveactivitytopreventthedegradationdegenerationphenomenon.SpacefproteinstructuremainlyrelyonhydrogenbondingsaltbondingtheVerWaalsbondingtheexistenceoftheeventacidalkalitreatmenthightemperatur

3、eMechanicaldramaticrolestrongradiationcanleadtothelossofactivityitshouldchoosetheconditionsftheverymodest.SDSPAGEastheisolationidentificationofbiologicalmacromoleculesinoneoflabatymeansithasbecomeoneoftheimptantlabatytec

4、hniqueinMolecularbiology.Thisexperimentusesthreedifferentmethods(twomethodsareproteinstodissolvesolutiononemethodisproteinprecipitation)withdrawsthetissuesproteinofArabidopsisthaliana(alltheproteinofArabidopsisthalianath

5、eleafproteinofArabidopsisthalianatheinjuriestissueproteinofArabidopsisthaliana)thentheproteinatlasindifferenttissuesgansofArabidopsisthalianawereanalyzedwithSDSpolyacrylaegelthenthroughtheanalysisoftheSDSPAGEpicturewecan

6、analysisthedifferentextractbufferthedifferentproteinofthedifferentganizations.Finallytheresultsindicatethatthebestmethodisextractbuffer2—theSDSPAGEpictureistheclearesttheextractbuffer3thatisthetrichloaceticacidacetonenex

7、tthelastistheextractbuffer1whichisPBS.ThekindsofproteininthewholeArabidopsisthalianaweremostclearestinthesethreetissuesthekindsofproteinintheleafwereleastthisphenomenoncanbeexplainedbythattheproteinscontenttheproteinstyp

8、earedifferentthisindicatesthatthenumberofthestripbeltbhastherelevancewiththefunctionnaturethephenomenonthatthenumberoftheleafproteinistheleastindicatethatthecontenttypeoftheleafproteinareonlyapartofthewholeproteinofArabi