博萊霉素刺激大鼠肺泡巨噬細胞對kv1.3和kv1.5通道m(xù)rna與蛋白表達的影響

1、華中科技大學碩士學位論文博萊霉素刺激大鼠肺泡巨噬細胞對Kv1.3和Kv1.5通道m(xù)RNA與蛋白表達的影響姓名：沈大紅申請學位級別：碩士專業(yè)：呼吸內科指導教師：陶曉南2011-05-17華 中 科 技 大 學 碩 士 學 位 論 文華 中 科 技 大 學 碩 士 學 位 論 文 2Effects of the mRNA and protein expression of Kv1.3 and Kv1.5 channels in rat ma

2、crophages cell by bleomycin Department of Respiratory Medicine, Union Hospital of Tongji Medicine College, Huazhong University of Science and Technology Postgraduate Shen Dahong Tutor Prof. Tao Xiaonan Abstract Obje

3、ctive To investigate the expression of Kv1.3 and Kv1.5 channels in rat macrophages cell line NR8383 cells under the stimulation of bleomycin. Methods: Cultured the NR8383 cells in vitro. Then,(1) five grouped cells were

4、treated with BLM in five different drug concentrations ( 0、 0.1、 1.0、 10、 100 mU/ml ); (2) treated cells in four groups with NS, DEX, DEX+BLM and BLM, respectively. Following above, detected the mRNA and protein of Kv1.3

5、 and Kv1.5 channels by Real time PCR and Western blot. Results: (1) Kv1.3 and Kv1.5 expressed in the NR83683 cells. (2) Compared with the control group, the expression of Kv1.5 channels increased under the stimulation of