川芎嗪對糖尿病腎病大鼠腎組織TGF-β1和CTGF表達干預的實驗研究.pdf

1、成都中醫(yī)藥大學碩士學位論文川芎嗪對糖尿病腎病大鼠腎組織TGFβ1和CTGF表達干預的實驗研究姓名：韓斌申請學位級別：碩士專業(yè)：中醫(yī)內科學指導教師：謝春光200705成都中醫(yī)藥大學碩士學位論文2ABSTRACTObjective：ByobservingtheeffectofLigustrazineonthepathologicalchangesinkidneyorganizationofDiabeticNephropathyratsand

2、theexpressionofTGFβ1、CTGFinvestigatethemechanismofLigustrazineinpreventingdiabeticglomerularsclerosisandinterstitialfibrosis.Method:AftercreatingtheDNmodelsofSDratsdividedtheanimalsintofourgroupsrandomly:includingmodelgr

3、oup，westmedicinegroup，Ligustrazine(highandlowdosage)groups.TakeanotherSDratscutrightkidneyasnormalgrouptotalfivegroups.Theratsoftreatinggroupsareaffusedinthecorrespondingmedicinetheotherareaffusedinthesamevolumewaterduri

4、ng12weeks.TestUAERinthe4th8th12thweekrespectivelyandmeasurthelevelofBUN、Scr、TC、TGinserumofeverygroup12weekslaterthenkillthemcuttheleftkidneysfixobservethepathologicaltransformationofrenalglomerulus.TheCTGFandTGFβ1aredete

5、ctedbyimmunohistochemistryandstatisticalanalysis.Result:Comparingwiththenormalgroup，thelevelofTG、TCandUAER、BUN、Scrofthemodelgrouprosecontinuously(P＜0.01；P＜0.05).TheexpressionofCTGFandTGFβ1inrenalorganizationraisedsignifi

6、cantly(P＜0.01).LigustrazinehighdosagehadtheroleofgettingTG、TCandUAER、BUN、ScrdecreasedandstepdowntheexpressionofCTGFandTGFβ1inrenalinterstitial(P＜0.05).Conclusion:Ligustrazinecoulddecreasethedrainageofuricproteinpreventth

7、erenalinterstitialfibrosispostponetheprocessofDN.TurningdowntheTGandregulatingtheexpressionofTGFβ1、CTGFinkidneymaypossiblybeoneofitsfunctionalmechanisms.Keyword:LigustrazineDiabetesNephropathyThekidneyOrganizationTGFβ1CT