產(chǎn)雙酶假單胞桿菌的誘變選育及發(fā)酵條件的優(yōu)化.pdf

1、”，：I?！猰——【1口＆——一t㈣I；15=』M一中南林業(yè)科技大學(xué)學(xué)位論文指導(dǎo)救■妊2：——盟—旦』生—jL_圭t———一生盤掛些盤掛盤生』且』遜業(yè)!i—o請(qǐng)咩位蛆別————巫——主————一——學(xué)剎、專業(yè)2赫：韭盟—翌———————，1競(jìng)方向——且—盟—叢—生—墊—————一論文答辯日期：』業(yè)￡生旦d沿置提交12期。業(yè)必盟學(xué)位樁干單位和日期—主蟲(chóng)堡些盤撞盤生—!旦旦出—且菩$圣m喪王席：——，—生一薹—型丑蠱且_一產(chǎn)雙酶假單胞桿菌

2、的誘變選育及發(fā)酵條件的優(yōu)化ABSTRACTDPhenylglycinewhichcanbeusedtoproduce131actamantibioticsandcephalosporinlikeamoxicillincteisakindofmostimportantpl“ecugsorItisusuallyproducedthroughthreewaysbutthemosteconomicwayisproducingbybiocnzym

3、ePseudomonaspuadawhichisresearchedinthisdissertationcanexcretetwoenzymesatthesametime：DhydantoinaseandDcarbamoylaseThesetwoenzymescancatalyseDhydrocyphenylhydantointoD—PhenylglycinedirectlythewildPseudomonasputidaproduce

4、slowenzymeactivitythestudymainlyinducedthewildstrainstoproducehighenzymeactivityTheindexesofstartingbacteriawasvalidatedThroughthecomparisonofdefinitionthegrowthculwewasmakeoutAndtheresultsshowed：thebest婦ofmutagenesiswas

5、after24hcultureofbactexinTheordc：rnessbetweenenzymeactivityandgrowthcurvewasanyalysedbyPDAB，whichshowedthattheenzymeactivitywouldbehigherattheendofthelogarithmtimeAndtheenzymeactivityofstartingbacteriawas0107p／mLwhichwas

6、measuredbyHPLCInthisstudyUV、MW、USandtheircombinationWeI℃usedtOmutagcnizethewildstrains，thenthenewbacteriawaKescreenedoutwithsubstrateanalogueFinallyseveralnewbacteriawerescreenedoutwhichexcretemo∞D(zhuǎn)hydantoinaseandDcarbamo

7、ylaseAmongthosenewbacteriathetotalenzymeactivityofFUMS06canreach0440肚whichenhanced31121％comparedwiththestartingbacteriaTheculturemodi啪andthefermentationconditionswereoptimizedbasedontheformerculturemeg[imandformalfermate

8、tionconditionsTheresultsshowedthattheenzymeactivityofFUMS06enhancedmoreAndthebestcompositionwasasfollowing：cornsteepliquor3O％，glucose25％，revuⅢse毗35％NaCl03％TheFUMS06grewwell011theoptimizedmediumcompared稍ththeformalmediumT

9、heenzymeactivityenhanced118％Thebestfermentationconditionwasasfollowing：Theinoculumamount10％Joadingamount40mLculmretime26hinitialpH74ThefinallyresultoffermentationwasidealicandtheactivityofFUMS06canremainafterseveralgener