中醫(yī)不同治則代表方藥對(duì)胃癌細(xì)胞端粒酶活性及細(xì)胞凋亡的影響.pdf

1、分類號(hào)密紐：?jiǎn)挝淮a學(xué)號(hào)10441山r虧中醫(yī)藹天虧碩士學(xué)位論文論文題目中醫(yī)不同治則代表方藥對(duì)胃癌細(xì)胞端粒酶活性及細(xì)胞凋亡的影響@研究生姓名入學(xué)年月學(xué)科專業(yè)指導(dǎo)教9ip學(xué)位類型李昕2003年9月中西醫(yī)結(jié)合基礎(chǔ)聶克醫(yī)學(xué)科學(xué)學(xué)位2006年4月20日EmctofTraditionalChineseMedicineonBGCceH’sTelome豫seActivityandApoptosisSpeciality：BasicResearchofIn

2、tegratedTCMandWesternMedicineAuthorLiXinTutor：NieKeAbstractobjegtlve：Cancerisamajordiseasewhichaffectspeople’shealthandlifeseverelyTheTraditionalChineseMedicinehasaninimitableeffectontumorOneoftheTraditionalChineseMedici

3、ne’scharacteristicisdiagnosisandtreatmentbasedontheoverallanalysisofsymptomsandsigns，whichmeansherbalistdoctorsmaychoosequitedifferenttherapeuticprinciplesandthehomologousprescriptionsbasedondifferentsymptomsandsignsdesp

4、itethesametumorAnd，theyusuallygainthewellpleasingeffectBut，sofarthemechanismhasn’tbeenclarifiedInordertoexplorethemechanism，thisstudyresearchedseveralprescriptionsbyusingFlowCytometerandSerumPharmacologyMaterialsandMetho

5、ds：WistarratsweredividedrandomlyintoeightgroupsTheratsineachgroupweretookdifferentdecoctionstopreparepharmicratserumThentheBGC—cellswereculturedinusingtheratSelalmNextindexessuchasMTrapoptosisandtelomeraseactivityweretes

6、tedandanalysedResults：(1)Compared、vitllnormalgroup，pharmicserumgroupsshowedsignificantinhibitorygrowthofBGCcellsAndtheinhibitionisremarkablyincreasedasweincreasetheconcentrationorprolongthetime(2)Therewassignificantincre

7、aseofapoptosisrateasweaffiliatethepharmicserumtothecells’system(3)ThelevelsoftelomeraseactivitywereloweraftertheBGCcellswereculturedinpharmieserumConclusions：pharmicserumgroupsshowedsignificantdiferrenceintheareaofinhibi