日本七鰓鰻白細胞轉(zhuǎn)錄組分析及免疫相關(guān)基因的比較研究.pdf

1、遼寧師范大學碩士學位論文日本七鰓鰻白細胞轉(zhuǎn)錄組分析及免疫相關(guān)基因的比較研究姓名：張寧申請學位級別：碩士專業(yè)：細胞生物學指導教師：李慶偉20100401日本七鰓鰻白細胞轉(zhuǎn)錄組分析及免疫相關(guān)基因的比較研究IILampetrajaponicaleukocytestranomicanalysiscomparativestudyofimmunerelatedgeneAbstractInalongtimetheiginevolutionofthea

2、daptiveimmunesystemhasbeenthefocusoftheimmunologyresearch.Thelateststudiesconfirmthetruththatthejawlessvertebrates(lampreyshagfishes)areattheinterfacebetweentheinnateimmunesystemtotheadaptiveimmunesystem.Recentfindingsin

3、thejawlessvertebrateshaverevealedthatitpossessessomealternativeimmunemoleculestheemergenceofadaptiveimmunityreaction.Thesespeciesareconsideredkeystounderstingthetheiginevolutionofadaptiveimmunity[1].InthispaperAcDNAlibra

4、rywasconstructedfromthemononuclearleukocytesextractedfromperipheralbloodofLampetrajaponicawhichwereimmunizedwithantigencomplexmethan10500romlyedclonesweresequenced.ThesesequenceswerecontigedbyPHRAPsoftware.Theresultsdemo

5、nstratedthereare1080consensussequences2302uniquetrans59.08％transsharedsimilaritytosequencespublishedinNCBIdatabases.ThefunctionalannotationstoassembleThestatisticaldataindicatedtheseESTswithsequencesdepositedinthedatabas

6、esledtotheidentificationofnumerousgeneshomologoustothosepredominantlyacteristicallyexpressedinmammalianlymphocyteswhichincludedgenescontrollingcelltranionproteinsynthesisinvolvementbiotranspotationprocess.Genescloselyrel

7、atedtothosethatingnathostomescontrollifeprocessingsuchastranionelongationfactadeninenucleotidetranslocatribosomalproteinreversetranasecytochromeCoxidaseheatshockprotein.Inadditiontheseencodinggenescrespondedtolocithatinj

8、awedvertebratesareessentialcellsurfacemarkeractivitymolecularintheearlydifferentiationstageoflymphocytes.TheseactivitiesincluderegulationofTBcellstimulationproliferation(Lck、Blnk、BtxCD45).Thepresenceofthesegenesinthelamp

9、reygenomesupptthenotionthatVLRAVLRBreceptsareexpressedbyseparatelymphocytepopulationsthedistinctiveexpressionprofilesfVLRAVLRBlymphocytesresemblethoseofmammalianTBcellsintheearlystageofgrowth.Thefindingsthussuggestthatdu

10、alrecognitionresponsearmswithintertwinedfunctionwerefundamentaltotheevolutionofadaptiveimmunityinvertebrates.InthisstudyWehavealsoexpledthecellsurfacemarkerssignaltransductionpathwaybyanalysingtheESTssequencescollectedin