石斛的離體培養(yǎng)及遺傳穩(wěn)定性的分子檢測.pdf

1、Y960851分類號：Q塑!2論文編號：墊Q3Q墊Q貴州大學(xué)2006屆碩士研究生學(xué)位論文密級石斛的離體培養(yǎng)及遺傳穩(wěn)定性的分子檢測學(xué)科專業(yè)：研究方向：導(dǎo)師：研究生：植物學(xué)植物生物技術(shù)文曉鵬宋常美中幽貴州貴陽2006年05月石斛的離體培養(yǎng)及遺傳穩(wěn)定性的分子檢測AbstractDendrobiumagenusfromOrchldaceaeisakindofeliteherbageplantsinChinesetraditionalmedici

2、ne，whichcharacterizedinmultiplefunctions，suchasinsenescenceretardingandcancer—preventing，eye—brighteningandbody—strenthering、immune—improvingetcTosatisfytheincreasingdemandofgermplasmconservation，micropropagation，aswella

3、sthefurtherexploitation，j力vitrocultureandgeneticstabilityevaluationtotheinvitromaterialsofthesegermplasmswerecarriedoutinthispresentstudyThemainresultsareasf01lows：1EstablishmentofasepticculturesforshootsegmentsAfterrins

4、ingwithtapwater，shootsegmentsofDendrobiumnobilewerefirstlydippedin70％ethanolfor30SSecondly，theyweresurface—sterilizedbyimmersionin：1)O1％(w／V)HgCl2for12min：2)01％(w／v)HgCl2with35dropsofTween80for10minTheeffectofthetwomenth

5、odswerequitedifferent，andcontaminationratewere733％and167％，respectivelyThentheshootsegmentswereinoculatedinMSBA(10mg／L)NAA(04mg／L)sucrose(3O％)agar(O7％)forgerminationConsequently，invltroshootsweretransferredtoMSBA(12mg／L)N

6、AA(O4mg／L)sucrose(3O％)agar(07％)formultiplicationviaproto—corngenesis繃SNAA(O5mg／L)IBA(O5mg／L)sucrose(20％)agar(07％)gavethebesteffectforstreatheningseedlingFollowingashootelongationstage煳SNAA(10mg／L)sucrose(2O％)agar’(07％)sh

7、owedthebesteffectonrootingOneortwopineconeswereputtedintheoneoffplasticcupwithsomeholesandthenlayedconsiderablelichenorbarkontheinvitroplantsshowedhigherthan90％ofsurvival凹vifroaftertranplanting2Callusinductionandregenera

8、tionInvltroshootsegmentsofDendrobiumnobilewereemployedtoinducecal1usDifferentbasalmedium，typesandconcentrationofplantgrowthregulators(PGRs)diverselyshowedcallusformationabilityTheodersofcallusformationrateisasfollows：1)m