擬南芥ATAF1轉錄因子在干旱脅迫響應中的功能分析.pdf

1、中國農業(yè)大學博士學位論文擬南芥ATAF1轉錄因子在干旱脅迫響應中的功能分析姓名：陸平利申請學位級別：博士專業(yè)：植物學指導教師：王學臣20061101AbstractDroughtisamongthemostimportantenvironmentalfactorsinfluencingplantgrowthdistributionanddevelopmentPlantshaveevolvedvaflougsurvival柚∞kmi蜘叫t

2、ocoml地tmntinucda‘p嗍刪峙todroughtst=ssincluding砒∞鯽磚i卸a】transdu砸oawhichleadstovoriottsphysiologicalandmetabolicresponsessuch鹋stre∞responsiveg衄eexpressionTranscriptionfactorsphyanimportantroleinthestresssignalnansduc婦byregula

3、tingtheexpressionofstressfunctionalgenesInArabidopsismany仃lm∞rip婦factorssuch艄AP2／EREbasicl印_cinezippers，NACMYB，MYCandseveredcla88簋ofzincfingerd餌nains，havebernhnpUcatedinplantdrought就【粥m印刪l瞄Ourworksidentifiedtnovddroughts

4、altandABAinduciblege日e’ATAFI，伽cod群NAC黼ly班o￡cinm越negativdy地鰣l岫the矗p瑚麗蛐ofstressresponsivegenesunderdroughtstressinArabidopsisNACproteh擔玳plantspecificⅡ：蜘∞蝻pt|onalregulatorsTheNACdomainwasofiginany曲刪哥ct盯幻edfromtheNAM(NoApica

5、lMeristem)sequencefrompetuniaeadtheATAFl，ATAF2andCUC2(cupshapedcotyledon)genrefromArabldopsisAlthoughATAFIⅥ∞oneofthefirstNACdomainproteinsidentifiedinArabidopsisitsbiologicalfunction吼謝remainsunI口10w也Inpresentstudy骶ch扣翟d電

6、ri∞dthcATAFIe曩preuionandbiological矗md曲旺inresponsetowaterdeficits懈sNorthernblotanalysisindicatedthatATAFImRNAexpressionwasstronglyinducedbydehydrationandhi【ghsalttreatmentbutinhibitedbyWarel“treatmentsuggesting8generalrol

7、eindrought“∞嘲responsl镕FurtherquantitativerealtimePCRresultsshowedthatitisalsoinducedbyexogenousABAtreatmentHistochemicalanalysisof阻glucuronidascactivitydrivenbytheATAFIpromot口demonstratedthatATAFIjsa弘岱sedthroughoutthepla

8、ntwithpredominant“pl岱si∞instomaUd刪cellsandv88allarsystemsFurthecanalysissuggestedthatATAFIpromoterisad∞ughtsaltandABAindudble班偽m慨TransientexpressionanalysisinonionepidermalcellsindicatedthenuclearlocalizationfortheATAFI：

9、：GFPfusionproteinYeasttransacfivalionanalysisshowedthatATAFIhadability協(xié)activatereportergeneeⅨprcssionFurthamoredomaindeletionanalysisrewealedth越theATAFItransactivationactivitywasconferredbyitsCterminaldomahlWhenATAFlgene

10、WaSknockedoutby’TDNAinsertions，Arabidopsis口蛔療jandatafl2mutantsdisphyedarecoveryrateabout船眥timeshigherthanwildtypcpl∞缸indroughtrespon目gtestToinvestigatethemolecularmechaaisms們analyzedtheeapressionofsomesRe∞responsivematke