皂莢抗心肌缺血活性成分篩選及機理研究.pdf

1、成都中醫(yī)藥大學博士學位論文皂莢抗心肌缺血活性成分篩選及機理研究姓名：吳建明申請學位級別：博士專業(yè)：中藥學指導教師：黃國鈞200804成都中醫(yī)藥大學二○○五級博士研究生學位論文第2頁AbstractAbstractGleditsiasinensisLam.wasextensivelyusedintheclinicalpracticesofTraditionalChineseMedicine.Itsantimyocardialischemi

2、aeffectsthemechanismswerestudiedbythephytochemicalpharmacologicalthemolecularmethodsinthethesisaccdingtoclinicalexperiencestheTradionalChineseMedicialtheies.Theresultsshowedas:FirstlytheGleditschiasaponinhadtheantimyocar

3、dialischemiaeffectswhichwasnotasgoodasthatoftheGleditschiasapogenin.TheresultsuggestedthattheGleditschiasapogeninwasthemainlyactivefractionoftheGleditschiasaponininvivo，accdingtotheiesofintestinalflametabolismserumpharma

4、cologicalstudy.SecondlytheGleditschiasapogeninmainlyincludetheechinocysticacid(EA)theoleanolicacid(OA)bothofwhichhadthesimilarantimyocardialischemiaactivity.ItwasthefirsttimethattheEAtheOAwerereptedasthemaincomponentswit

5、hantimyocardialischemiaactivityofGleditsiasinensisLam.ThirdlyahighperfmanceliquidchromatographicmethodhadbeenestablishedtostudythebioavailabilityoftheEA.Thequantitativelimitationlinearityrepeatabilityofthemethodweresatis

6、facty.FourthlytheCaCo2cellmodeltheratpharmacokiicmodelwereemployedtocomparethebioavailabilityoftheEAitssodiumsalt.TheresutsrevealedthatthesodiumsaltofEAhadthebetterbioavailabilitythanEA.Fifthlytheantimyocardialischemiame