生物微膠囊固定化溶栓酶產生菌及釀酒酵母微囊化培養(yǎng)模型化的初步研究.pdf

1、浙江大學碩士學位論文生物微膠囊固定化溶栓酶產生菌及釀酒酵母微囊化培養(yǎng)模型化的初步研究姓名：姚勁挺申請學位級別：碩士專業(yè)：生物化學工程指導教師：梅樂和2001.1.1浙江大學碩士學位論文ABSTRACTABSTRACTFreeandimmobilizedcultivationofBacillussubtilisgenu(ZJUBETI)whichcanproduceantithrombusenzyme(ATE)wasstudiedAfun

2、damentalmathematicmodelaboutproductionofethaneIbySaccharomyccseerevisiaeimmobilizedinNaCSPDMDAACmicrocapsuleswasestablishedTheinfluenceoffreecultureonproductionofATEandgrowthofbacteriawereinvestigatedWhenglucose(05％)Wasa

3、ddedintoLBculturemedium，thepropagationofbacteriaWasprohibitedbecausepHdecreasedsignificantlyTheoptimumconcentrationofphosphateWasO30％(NaI12P042H20)and144％(Na2HP0412H20)Basedontheconcen仃afionofbufferahead，theoptimumconcen

4、trationofglucoseWas05％ThecharacterofgrowthofthegenuWasstudiedwhenammoniBillsulfateorureawasusedasnitrogensoRreeWhenhydrolyzedtryptoneservedasni仃ogensource，也eenzymeactivityreached757UK枷ThecharacterofproductionofATEbythege

5、nuencapsulatedinNaCSPDMDAACmicrocapsuleswasstudiedTherelationshipbetweenairvolumnvelocityandmeandiameterofmicrocapsulesintheprocessofproducingmicrocapsulesWasstudiedfirstiyThentheinfluenceofmediumdiameterofmicrocapsulesc

6、oncentrationofPDⅣ【DAACandthecomponentsofculturemediumonproductionofA：rEintheconditionofencapsulationcultureWasstudiedInshakingflasks，themaximumenzymeactivityinmicmcapsulesWas1850UK／111l(urokinase卸油i鉀uniteachmilliliterbro

7、thinmicrocapsules)whentheairvohmmvelocityWas03m3／h(themeandiameterofmicrocapsulesWas160ram)theconcentrationofPDMDAACwas20％andtheculturemeAiumwasLBculturemedium(tryptone)glucose(o。5％)phosphatebuffer(100m1)Abubblingculture

8、systemWasdesignedInthissystem，themaximumenzymeactivityinmicrocapsulesreached2050lJ剮m1whichWas36timesthatinsuspensionfermentation；themaximumeelldensityinmicrocapsuleswas489109cell／m1whichWas27timesthatinsuspensionfermenta

9、tionAsimplemathematicmodeldescribingproductionofethanolbySaccharomycescerevisiaeimmobilizedinNaCSPDMDAACmicrocapsulesWasestablishedn地mainreasonsthatcauseddeviationbetweenexperimentalvalueandcalculatedValliewerediscusseda